Elena P P, Kosina-Boix M, Moulin G, Lapalus P
Invest Ophthalmol Vis Sci. 1987 Aug;28(8):1436-41.
Using an in vitro autoradiography, beta-adrenergic receptors were localized in the rabbit eye. Autoradiograms were generated by apposition of isotope-sensitive film to slide-mounted eye sections, labelled with 125IIodocyanopindolol. A high density of silver grains was obtained in conjunctival, corneal and ciliary process epithelium. Binding sites were also present in corneal endothelium, iris epithelium, lens epithelium, choroid and extraocular muscles. In some areas, retina was also labelled. Studies with beta-adrenergic compounds showed that the majority of beta-adrenergic receptors, detectable by autoradiography, were of the beta2 type in the rabbit eye.
利用体外放射自显影技术，在兔眼中定位了β-肾上腺素能受体。通过将同位素敏感膜与载于载玻片上的眼组织切片贴合，用[125I] - 碘氰吲哚洛尔进行标记，生成放射自显影片。在结膜、角膜和睫状突上皮中获得了高密度的银颗粒。角膜内皮、虹膜上皮、晶状体上皮、脉络膜和眼外肌中也存在结合位点。在某些区域，视网膜也有标记。对β-肾上腺素能化合物的研究表明，通过放射自显影可检测到的大多数β-肾上腺素能受体在兔眼中为β2型。
