Elena P P, Denis P, Kosina-Boix M, Saraux H, Lapalus P
Department of Pharmacology, Faculte de Medecine, Nice, France.
J Ocul Pharmacol. 1990 Summer;6(2):143-9. doi: 10.1089/jop.1990.6.143.
Beta adrenergic binding sites were localized and characterized in the human eye by means of "in vitro" autoradiography, using [125I] (-) iodocyanopindolol (125ICYP) as radioligand. Binding sites were visualized by apposition of isotope sensitive film to slide mounted eye sections. Receptor sites were present in the extraocular muscles, in the conjunctiva, in the epithelium and endothelium of the cornea, in the trabeculum and in the ciliary muscle. They were also present in the lens epithelium and in the retina. The pigmented ocular structures were heavily labelled but the binding was nonspecific. Characterization of these binding sites was achieved by testing the ability of selective adrenergic compounds to displace 125ICYP binding. These studies suggested that the majority of adrenergic binding sites in nonpigmented structures of human eye were of a beta2 type.
通过“体外”放射自显影术，使用[125I]（-）碘氰吲哚洛尔（125ICYP）作为放射性配体，对人眼中的β肾上腺素能结合位点进行了定位和表征。通过将同位素敏感膜与载于载玻片上的眼组织切片贴合来观察结合位点。受体位点存在于眼外肌、结膜、角膜上皮和内皮、小梁和睫状肌中。它们也存在于晶状体上皮和视网膜中。有色素的眼部结构被大量标记，但这种结合是非特异性的。通过测试选择性肾上腺素能化合物取代125ICYP结合的能力来实现对这些结合位点的表征。这些研究表明，人眼非色素结构中的大多数肾上腺素能结合位点是β2型的。
