嗜热凝结芽孢杆菌 IPE22 一步法敞开发酵生产不可食用淀粉生物质乳酸。
One step open fermentation for lactic acid production from inedible starchy biomass by thermophilic Bacillus coagulans IPE22.
机构信息
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, People's Republic of China; University of the Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing 100049, People's Republic of China.
State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100190, People's Republic of China.
出版信息
Bioresour Technol. 2019 Jan;272:398-406. doi: 10.1016/j.biortech.2018.10.043. Epub 2018 Oct 21.
The aim of this study was to establish a simplified operational process for lactic acid (LA) production by Bacillus coagulans IPE22 from inedible starchy biomass with open fermentation method. First, 29.47 mU/mg specific amylase activity was detected in direct batch fermentation from soluble starch, but the activity of the produced amylase was too low for effective production of LA. Then seven batches from 72 g/L soluble starch were conducted without sterilization. It was found that one step simultaneous liquefaction, saccharification and fermentation (SLSF) with the addition of mesothermal α-amylase and glucoamylase was the optimal mode with LA concentration, yield and productivity of 68.72 g/L, 0.99 g/g and 1.72 g/L h respectively. Finally, inedible starchy biomass, cassava and sorghum flours, were proved to be alternatives to refined soluble starch. For the first time, one step open SLSF of inedible starchy biomass was reported for LA production by B. coagulans.
本研究旨在建立一种简化的操作流程，使用凝结芽孢杆菌 IPE22 通过开放式发酵方法从不可食用的淀粉生物质生产乳酸（LA）。首先，在直接分批发酵中从可溶性淀粉中检测到 29.47 mU/mg 的特定淀粉酶活性，但产生的淀粉酶活性太低，无法有效生产 LA。然后在没有灭菌的情况下进行了七批从 72 g/L 可溶性淀粉开始的发酵。结果发现，添加中温α-淀粉酶和糖化酶的一步同时液化、糖化和发酵（SLSF）是最佳模式，LA 浓度、得率和生产强度分别为 68.72 g/L、0.99 g/g 和 1.72 g/L·h。最后，证明不可食用的淀粉生物质、木薯粉和高粱粉可以替代精制可溶性淀粉。首次报道了凝结芽孢杆菌一步法开放式 SLSF 从不可食用的淀粉生物质生产 LA。
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