Scarfe Lauren, Schock-Kusch Daniel, Ressel Lorenzo, Friedemann Jochen, Shulhevich Yury, Murray Patricia, Wilm Bettina, de Caestecker Mark
Division of Nephrology, Department of Medicine, Vanderbilt University Medical Center; Department of Cellular and Molecular Physiology, University of Liverpool.
MediBeacon GmbH;
J Vis Exp. 2018 Oct 21(140):58520. doi: 10.3791/58520.
Transdermal analysis of glomerular filtration rate (GFR) is an established technique that is used to assess renal function in mouse and rat models of acute kidney injury and chronic kidney disease. The measurement system consists of a miniaturized fluorescence detector that is directly attached to the skin on the back of conscious, freely moving animals, and measures the excretion kinetics of the exogenous GFR tracer, fluorescein-isothiocyanate (FITC) conjugated sinistrin (an inulin analog). This system has been described in detail in rats. However, because of their smaller size, measurement of transcutaneous GFR in mice presents additional technical challenges. In this paper we therefore provide the first detailed practical guide to the use of transdermal GFR monitors in mice based on the combined experience of three different investigators who have been performing this assay in mice over a number of years.
肾小球滤过率（GFR）的经皮分析是一种成熟的技术，用于评估急性肾损伤和慢性肾病小鼠及大鼠模型的肾功能。测量系统由一个小型荧光探测器组成，该探测器直接附着在清醒、自由活动动物背部的皮肤上，测量外源性GFR示踪剂异硫氰酸荧光素（FITC）偶联的左聚糖（一种菊粉类似物）的排泄动力学。该系统已在大鼠中得到详细描述。然而，由于小鼠体型较小，在小鼠中测量经皮GFR存在额外的技术挑战。因此，在本文中，我们基于三位不同研究人员多年来在小鼠中进行该检测的综合经验，首次提供了使用小鼠经皮GFR监测仪的详细实用指南。
