Department of Plant Sciences, Quaid-i-Azam University Islamabad, Islamabad, Pakistan.
Institute of Plant Sciences, University of Sindh Jamshoro, Sindh, Pakistan.
Microsc Res Tech. 2019 Sep;82(9):1410-1418. doi: 10.1002/jemt.23293. Epub 2019 May 20.
This was the first comprehensive pollen micromorphological investigation of lactiferous flora (Apocynaceae) of District Rawalpindi, Pakistan. The pollen morphology of 10 species of the family Apocynaceae was observed and documented using light microscopy and scanning electron microscopy. Pollen was found subspheroidal in shape in most of the species, however peroblate shape was noted in Vinca major. Exine sculpturing patterns (psilate, rugulate, scabrate, and microreticulate) were observed. The result indicated that exine ornamentation of Apocynaceae taxa is systematically informative at generic and species levels. Most of the species have tricolporate type pollen but tetraporate pollen was also observed in Trachelospermum jasminoides and tetracolpate in V. major. Minimum equatorial diameter was noted in Carissa edulis (27.13 μm) and maximum in V. major (108.25 μm). Similarly, maximum exine thickness was found in Cascabela thevetia (9.5 μm). In the present findings, the pollen morphological data are compared with available other pollen studies to evaluate the taxonomic value of pollen traits in Apocynaceae taxa by using multiple microscopic techniques. Furthermore, molecular and phylogenetic studies were recommended to strengthen the systematics of Apocynaceae taxa.
这是对巴基斯坦拉瓦尔品第地区乳源植物（夹竹桃科）的首次全面花粉微形态学研究。使用光学显微镜和扫描电子显微镜观察和记录了该科 10 种植物的花粉形态。大多数物种的花粉呈近球形，但长春花的花粉呈扁球形。观察到外壁纹饰（光滑、粒状、粗糙和微网状）。结果表明，夹竹桃科的外壁纹饰在属和种水平上具有系统信息。大多数物种具有三孔花粉，但在络石和长春花中也观察到四孔花粉，在长春花中也观察到四孔花粉。Carissa edulis 的赤道直径最小（27.13μm），长春花的最大（108.25μm）。同样，Cascabela thevetia 的外壁最厚（9.5μm）。在本研究中，将花粉形态数据与现有的其他花粉研究进行了比较，通过使用多种显微镜技术评估了花粉特征在夹竹桃科分类中的分类价值。此外，还建议进行分子和系统发育研究，以加强夹竹桃科分类群的系统发育。
