Hunan Key Laboratory of Grain-Oil Deep Process and Quality Control, Hunan Key Laboratory of Processed Food for Special Medical Purpose, College of Food Science and Engineering, National Engineering Laboratory for Deep Process of Rice and Byproducts, Central South University of Forestry and Technology, Changsha, Hunan 410004, China.
School of Life Science and Technology, Nanyang Normal University, No. 1638 Wolong Road, Nanyang, Henan 473061, China.
Bioresour Technol. 2019 Jan;272:552-560. doi: 10.1016/j.biortech.2018.10.072. Epub 2018 Oct 29.
A mutant Monascus purpureus strain, M183, which produced monascus pigments (MPs) at 8460 U/g via solid-state batch-fermentation, was generated using the atmospheric and room temperature plasma (ARTP) mutation system. The optimal glucose concentration (80 g/L) in traditional fermentation media that yielded the highest MPs productivity was determined. Response surface methodology (RSM) was applied to maximize MPs production using liquid-state batch-fermentation. Under optimal conditions (0.58 g/L MgSO·7HO, 0.02 g/L ZnSO·7HO, 0.02 g/L FeSO·7HO and 4.85 g/L NHNO), 207.67 U/mL of MPs were produced with 98.12% validity based on the predicted value. Extracellular MPs production increased significantly to 35.52 U/mL, compared to 14.19 U/mL of the original strain, M. purpureus LQ-6. M. purpureus spores immobilized in sodium alginate were studied. A simultaneous fermentation and separation system was established for MPs using the novel absorption resin LX300C to enhance production efficiency of extracellular MPs.
一株产色素能力为 8460U/g 的高产突变红曲霉菌株 M183 是利用大气压室温等离子体（ARTP）诱变系统选育得到的。确定了传统固态分批发酵生产红曲色素（MPs）的最佳葡萄糖浓度（80g/L）。采用响应面法（RSM）对液态分批发酵进行优化，以最大限度地提高 MPs 的产量。在最佳条件下（0.58g/L MgSO·7HO、0.02g/L ZnSO·7HO、0.02g/L FeSO·7HO 和 4.85g/L NHNO），根据预测值， MPs 的产量为 207.67U/mL，有效率为 98.12%。与原始菌株 M. purpureus LQ-6 的 14.19U/mL 相比，胞外 MPs 的产量显著增加到 35.52U/mL。研究了固定在海藻酸钠中的红曲霉菌孢子。利用新型吸附树脂 LX300C 建立了 MPs 的同步发酵和分离系统，以提高胞外 MPs 的生产效率。
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