El-Sayed El-Sayed R, Mousa Shaimaa A, Strzała Tomasz, Boratyński Filip
Department of Food Chemistry and Biocatalysis, Wrocław University of Environmental and Life Sciences, Norwida 25, Wrocław, 50-375, Poland.
Plant Research Department, Nuclear Research Center, Egyptian Atomic Energy Authority, Cairo, Egypt.
J Biol Eng. 2024 Aug 15;18(1):44. doi: 10.1186/s13036-024-00439-y.
Considerable attention has been paid to exploring the biotechnological applications of several Monascus sp. for pigment production. In this study, our focus is on enhancing the bioprocessing of red pigment (RP) derived from the endophytic fungus Monascus ruber SRZ112. To achieve this, we developed a stable mutant strain with improved productivity through gamma irradiation. This mutant was then employed in the immobilization technique using various entrapment carriers. Subsequently, we optimized the culture medium for maximal RP production using the Response Surface Methodology. Finally, these immobilized cultures were successfully utilized for RP production using a semi-continuous mode of fermentation. After eight cycles of fermentation, the highest RP yield by immobilized mycelia reached 309.17 CV mL, a significant increase compared to the original titer. Importantly, this study marks the first report on the successful production of Monascus RP in a semi-continuous mode using gamma rays' mutant strain, offering prospects for commercial production.
人们已经相当关注探索几种红曲霉菌株在色素生产方面的生物技术应用。在本研究中，我们的重点是提高源自内生真菌红曲红曲霉SRZ112的红色素（RP）的生物加工过程。为实现这一目标，我们通过伽马射线辐照开发了一种具有更高生产力的稳定突变菌株。然后，使用各种包埋载体将该突变体用于固定化技术。随后，我们使用响应面法优化了培养基以实现最大的RP产量。最后，这些固定化培养物成功地用于采用半连续发酵模式生产RP。经过八个发酵周期，固定化菌丝体的最高RP产量达到309.17 CV mL，与原始效价相比有显著提高。重要的是，本研究标志着首次报道使用伽马射线突变菌株以半连续模式成功生产红曲RP，为商业生产提供了前景。
