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位于[具体来源]呼吸复合体I中的基因8在细胞生长和次级代谢产物生物合成中的作用。

Role of the Gene 8 Located in Respiratory Complex I from in the Cell Growth and Secondary Metabolites Biosynthesis.

作者信息

Cai Xinru, Zhang Song, Lin Jia, Wang Yaxu, Ye Fanyu, Zhou Bo, Lin Qinlu, Liu Jun

机构信息

National Engineering Research Center of Rice and Byproduct Deep Processing, College of Food Science and Engineering, Central South University of Forestry and Technology, Changsha 410004, China.

出版信息

J Fungi (Basel). 2022 Jun 22;8(7):655. doi: 10.3390/jof8070655.

DOI:10.3390/jof8070655
PMID:35887413
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9319538/
Abstract

Our previous work revealed that the anabolism of secondary metabolites is closely related to cofactor metabolism. In this study, we have further investigated the regulation mechanisms of respiratory complex I in response to the cell growth and secondary metabolite biosynthesis of . The results showed that downregulating the mRNA level of gene 8 in sharply increased the secondary metabolites biosynthesis, cell growth and glucose consumption rates at the fermentation metaphase; slightly increased the colony diameter and biomass, and dramatically changed the mycelia morphology; and decreased the tolerances to environmental factors (especially HO). It also significantly inhibited the enzymes activities of respiratory complex I, III and superoxide dismutase, but stimulated that of complex II, IV and peroxidase, leading to an increase in reactive oxygen species (ROS) level and a decrease in ATP concentration. Furthermore, transcriptome analysis revealed that the mRNA levels of genes involved in respiratory chain, tricarboxylic acid cycle, and fatty acid degradation were downregulated, but those in the citrinin and pigment biosynthesis and related pathways were upregulated. These data revealed that complex I plays a vital role in regulating the cell growth and secondary metabolism of via changing the intracellular ROS and ATP levels.

摘要

我们之前的研究表明，次生代谢产物的合成代谢与辅因子代谢密切相关。在本研究中，我们进一步探究了呼吸链复合体I响应[具体对象]细胞生长和次生代谢产物生物合成的调控机制。结果显示，在发酵中期下调[具体对象]中基因8的mRNA水平，会显著提高次生代谢产物的生物合成、细胞生长及葡萄糖消耗速率；略微增加菌落直径和生物量，并显著改变菌丝形态；降低对环境因子（尤其是H₂O₂）的耐受性。它还显著抑制呼吸链复合体I、III和超氧化物歧化酶的酶活性，但刺激复合体II、IV和过氧化物酶的活性，导致活性氧（ROS）水平升高和ATP浓度降低。此外，转录组分析表明，参与呼吸链、三羧酸循环和脂肪酸降解的基因的mRNA水平下调，但桔霉素和[具体对象]色素生物合成及相关途径中的基因的mRNA水平上调。这些数据表明，复合体I通过改变细胞内ROS和ATP水平，在调控[具体对象]的细胞生长和次生代谢中起着至关重要的作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9312/9319538/efac917006bb/jof-08-00655-g013.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9312/9319538/efac917006bb/jof-08-00655-g013.jpg

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