a The Emergency Center, Gansu Nephro-Urological Clinical Center, Lanzhou University Second Hospital , Lanzhou China.
Ren Fail. 2018 Nov;40(1):618-627. doi: 10.1080/0886022X.2018.1496935.
To evaluate a Angelica sinensis polysaccharide aqueous extract as a preventive agent in experimentally induced urolithiasis using in- vitro and vivo models.
Angelica sinensis polysaccharide was investigated in vitro to determine its antilithiatic effects on the formation and morphology of calcium oxalate (CaOx) crystals and was analyzed in vivo to determine its ability to prevent CaOx urolithiasis in rats subjected to ethylene glycol-induced urolithiasis. Potassium citrate administration was used in the positive control group. The urolithiasis-related biochemical parameters were evaluated in the rats urine, serum and kidney homogenates. Kidney sections were subjected to histopathological and immunohistochemical analyses, and urolithiasis-related phospho-c-Jun NH2-terminal protein kinase and kidney injury molecule-1proteins were evaluated by Western blot analyses.
Angelica sinensis polysaccharide exhibited concentration-dependent inhibition of CaOx crystal formation. The in vitro assay revealed significant inhibition of crystal formation (6.99 ± 1.07) in the group treated with 4.0 mg/mL Angelica sinensis polysaccharide extract compared with the control group (58.38 ± 5.63; p < .05). In vivo, after treatment with ethylene glycol for 28 days, urinary oxidative stress, oxalate, creatinine, urea and urolithiasis-related protein were significantly increased (p < .05), except for serum oxidative stress (p > .05). The rats administered the extract of Angelica sinensis polysaccharide showed significantly decreased pathological change and CaOx deposition (p < .05) compared with the urolithiatic rats. Significantly reduced levels of urinary oxidative stress, oxalate, creatinine, urea and urolithiasis-related protein were observed in the Angelica sinensis polysaccharide treatment groups (p < .05) compared with the nephrolithic rats.
The results presented here suggest that Angelica sinensis polysaccharide has the potential to inhibit CaOx crystallization in vitro and may present anti-urolithiatic effects in vivo.
使用体外和体内模型评估当归多糖水溶液提取物作为实验性尿石症预防剂的作用。
在体外研究当归多糖对草酸钙（CaOx）晶体形成和形态的抗结石作用，并在体内分析其预防乙二醇诱导尿石症大鼠 CaOx 尿石症的能力。柠檬酸钾给药作为阳性对照组。评估大鼠尿液、血清和肾匀浆中的尿石症相关生化参数。对肾组织切片进行组织病理学和免疫组织化学分析，并通过 Western blot 分析评估尿石症相关磷酸-c-Jun NH2-末端蛋白激酶和肾损伤分子-1 蛋白。
当归多糖表现出浓度依赖性抑制 CaOx 晶体形成。体外试验显示，与对照组（58.38±5.63）相比，4.0mg/mL 当归多糖提取物处理组的晶体形成显著抑制（6.99±1.07）（p<.05）。体内，用乙二醇处理 28 天后，尿氧化应激、草酸盐、肌酐、尿素和尿石症相关蛋白显著增加（p<.05），除血清氧化应激外（p>.05）。与结石形成大鼠相比，给予当归多糖提取物的大鼠病理变化和 CaOx 沉积明显减少（p<.05）。与肾结石大鼠相比，当归多糖治疗组的尿氧化应激、草酸盐、肌酐、尿素和尿石症相关蛋白水平显著降低（p<.05）。
本研究结果表明，当归多糖具有体外抑制 CaOx 结晶的潜力，并可能具有体内抗尿石症作用。
