Li Cheng, Li Hongyan, Gilbert Robert G
Joint International Research Laboratory of Agriculture and Agri-Product Safety of Ministry of Education of China, Yangzhou University, Yangzhou, Jiangsu Province, China.
School of Food and Chemical Engineering, Beijing Technology and Business University, Beijing, China.
Methods Mol Biol. 2019;1892:169-185. doi: 10.1007/978-1-4939-8914-0_10.
A better understanding of the nutritional properties of rice starch is important because of the rapid rise of diet-related health complications, particularly obesity, type 2 diabetes, and colorectal cancers. Rice starch that is slowly digested to glucose, and where significant quantities of starch which reach the lower gut ("resistant starch"), can mitigate, and also delay the onset of, these diseases. These digestibility properties depend to some extent on starch molecular structure. The characterization of this structure is therefore significant for understanding and developing healthier slower digestible rice. In this chapter, a series of techniques used for characterizing starch structure are reviewed and the procedure for preparing rice starch samples with minimum degradation for characterizing starch chain length distribution (CLD) and overall molecular structure is given. Some methods for choosing or developing plants showing desirable structural characteristics are briefly summarized.
鉴于与饮食相关的健康并发症（尤其是肥胖症、2型糖尿病和结直肠癌）迅速增加，更好地了解大米淀粉的营养特性非常重要。缓慢消化为葡萄糖的大米淀粉，以及大量到达下消化道的淀粉（“抗性淀粉”），可以减轻并延缓这些疾病的发作。这些消化特性在一定程度上取决于淀粉的分子结构。因此，这种结构的表征对于理解和开发更健康、消化更慢的大米具有重要意义。在本章中，将对用于表征淀粉结构的一系列技术进行综述，并给出制备具有最小降解的大米淀粉样品以表征淀粉链长分布（CLD）和整体分子结构的程序。还简要总结了一些选择或培育具有理想结构特征植物的方法。
