Kuzichkina E O, Shilova O N, Deyev S M, Petrov R V
Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, 117997, Russia.
Moscow Institute of Physics and Technology (State University), Dolgoprudnyi, Moscow oblast, 141700, Russia.
Dokl Biochem Biophys. 2018 Sep;482(1):245-248. doi: 10.1134/S1607672918050046. Epub 2018 Nov 5.
It was found that the fluorescent properties of the phototoxic domain of miniSOG allow to assess the ability of toxins to bind to human breast adenocarcinoma cells SK-BR-3 and study the dynamics of their internalization. We established that the main cause of the decrease of the fluorescence intensity of the recombinant proteins 4D5scFv-miniSOG and DARPin-miniSOG during their internalization in the complex with the HER2 receptor is their shielding and absorption of the fluorescence of miniSOG by the cells fluorophores.
研究发现，微小光敏感蛋白（miniSOG）光毒性结构域的荧光特性可用于评估毒素与人类乳腺腺癌细胞SK-BR-3的结合能力，并研究其内化动力学。我们确定，重组蛋白4D5单链抗体片段-微小光敏感蛋白（4D5scFv-miniSOG）和设计锚蛋白-微小光敏感蛋白（DARPin-miniSOG）在与HER2受体形成复合物内化过程中荧光强度降低的主要原因是细胞内荧光团对微小光敏感蛋白荧光的屏蔽和吸收。
