Benson R C, Meyer R A, Zaruba M E, McKhann G M
J Histochem Cytochem. 1979 Jan;27(1):44-8. doi: 10.1177/27.1.438504.
For many cell types, cellular autofluorescence in the 500-600 nm spectral region can result in a significant background signal for measurements of weakly fluorescent probes. Measurements of fluorescence intensity and spectra of a variety of cell types and assays of neuronal homogenates are presented to demonstrate that this autofluorescence is most likely due to endogenous flavoproteins.
对于许多细胞类型而言，500 - 600纳米光谱区域的细胞自发荧光会在测量弱荧光探针时产生显著的背景信号。本文展示了多种细胞类型的荧光强度和光谱测量结果以及神经元匀浆的检测结果，以证明这种自发荧光很可能源自内源性黄素蛋白。
