State Key Laboratory of Plant Genomics, Institute of Microbiology , Chinese Academy of Sciences , Beijing , China.
Institute of Plant Protection , Xinjiang Agricultural Academy of Sciences , Xinjiang , Urumqi , China.
J Agric Food Chem. 2018 Nov 14;66(45):11990-11999. doi: 10.1021/acs.jafc.8b03914. Epub 2018 Nov 6.
RNA interference (RNAi) has been developed for plant pest control. In this study, hairpin-type double-stranded RNA (dsRNA) targeting the juvenile hormone (JH) acid methyltransferase ( JHAMT) gene ( dsJHAMT) was introduced in potato plants via Agrobacterium-mediated transformation. The results indicated that the transcriptional RNA of dsJHAMT accumulated in the transgenic plants. The transcripts and proteins of the L. decemlineata JHAMT gene were significantly reduced in larvae feeding on dsJHAMT transgenic foliage. The dsJHAMT had a significant negative effect on the growth and development of L. decemlineata, especially resulting in less oviposition. Importantly, in the field trials, transgenic plants are high-efficiently protected from insect damage mainly because surviving insects laid fewer or no eggs. Even full protection from beetle damage can be acquired by continuously lowering insect population size at large scale in the field over the years. Therefore, the transgenic plants expressing dsJHAMT successfully provided an additional option for plant pest control.
RNA 干扰 (RNAi) 已被开发用于防治植物害虫。在这项研究中，通过农杆菌介导的转化将靶向保幼激素 (JH) 酸甲基转移酶 (JHAMT) 基因的发夹型双链 RNA (dsRNA) 导入马铃薯植株中。结果表明，dsJHAMT 在转基因植物中积累了转录 RNA。取食 dsJHAMT 转基因叶片的幼虫中 L. decemlineata JHAMT 基因的转录物和蛋白质显著减少。dsJHAMT 对 L. decemlineata 的生长和发育有显著的负面影响，特别是产卵量减少。重要的是，在田间试验中，转基因植物能够高效地免受昆虫的侵害，主要是因为存活的昆虫产卵少或不产卵。甚至通过多年来在田间不断降低大规模昆虫种群数量，也可以获得对甲虫的完全保护。因此，表达 dsJHAMT 的转基因植物成功地为植物害虫防治提供了另一种选择。
