一种细胞内二胺氧化酶触发的超极化氙磁共振生物传感器。
An intracellular diamine oxidase triggered hyperpolarized Xe magnetic resonance biosensor.
机构信息
State Key Laboratory for Magnetic Resonance and Atomic and Molecular Physics, National Center for Magnetic Resonance in Wuhan, Wuhan Institute of Physics and Mathematics, Chinese Academy of Sciences, Wuhan National Laboratory for Optoelectronics, Huazhong University of Science and Technology (HUST), Wuhan 430071, China.
出版信息
Chem Commun (Camb). 2018 Dec 4;54(97):13654-13657. doi: 10.1039/c8cc07822j.
Here, a novel method was developed for suppressing 129Xe signals in cucurbit[6]uril (CB6) until the trigger is activated by a specific enzyme. Due to its noncovalent interactions with amino-groups and CB6, putrescine dihydrochloride (Put) was chosen for blocking interactions between 129Xe and CB6. Upon adding diamine oxidase (DAO), Put was released from CB6 and a 129Xe@CB6 Hyper-CEST signal emerged. This proposed 129Xe biosensor was then tested in small intestinal villus epithelial cells.
这里，开发了一种新方法来抑制葫芦[6]脲（CB6）中的 129Xe 信号，直到被特定的酶触发。由于其与氨基和 CB6 的非共价相互作用，腐胺二盐酸盐（Put）被选择用于阻断 129Xe 和 CB6 之间的相互作用。加入二胺氧化酶（DAO）后，Put 从 CB6 中释放出来，出现了 129Xe@CB6 Hyper-CEST 信号。然后，在小肠绒毛上皮细胞中测试了这种新的 129Xe 生物传感器。
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