使用超短饱和预脉冲序列通过HyperCEST检测全血中的葫芦[6]脲。
HyperCEST detection of cucurbit[6]uril in whole blood using an ultrashort saturation Pre-pulse train.
作者信息
Hane Francis T, Smylie Peter S, Li Tao, Ruberto Julia, Dowhos Krista, Ball Iain, Tomanek Boguslaw, DeBoef Brenton, Albert Mitchell S
机构信息
Lakehead University, Department of Chemistry, 955 Oliver Rd, Thunder Bay, ON P7B 5E1, Canada.
Thunder Bay Regional Research Institute, 980 Oliver Rd, Thunder Bay, ON P7B 5E1, Canada.
出版信息
Contrast Media Mol Imaging. 2016 Jul;11(4):285-90. doi: 10.1002/cmmi.1690. Epub 2016 Apr 13.
Xenon based biosensors have the potential to detect and localize biomarkers associated with a wide variety of diseases. The development and nuclear magnetic resonance (NMR) characterization of cage molecules which encapsulate hyperpolarized xenon is imperative for the development of these xenon biosensors. We acquired (129) Xe NMR spectra, and magnetic resonance images and a HyperCEST saturation map of cucurbit[6]uril (CB6) in whole bovine blood. We observed a mean HyperCEST depletion of 84% (n = 5) at a concentration of 5 mM and 74% at 2.5 mM. Additionally, we collected these data using a pulsed HyperCEST saturation pre-pulse train with a SAR of 0.025 W/kg which will minimize any potential RF heating in animal or human tissue. Copyright © 2016 John Wiley & Sons, Ltd.
基于氙的生物传感器有潜力检测和定位与多种疾病相关的生物标志物。对于这些氙生物传感器的开发而言，封装超极化氙的笼形分子的开发及核磁共振（NMR）表征至关重要。我们获取了全牛血中葫芦[6]脲（CB6）的（129）Xe NMR光谱、磁共振图像和HyperCEST饱和图。我们观察到，在5 mM浓度下平均HyperCEST损耗为84%（n = 5），在2.5 mM浓度下为74%。此外，我们使用比吸收率为0.025 W/kg的脉冲HyperCEST饱和预脉冲序列收集这些数据，这将使动物或人体组织中的任何潜在射频加热降至最低。版权所有© 2016约翰威立父子有限公司。
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