Department of Oral Medicine and Oral Diagnosis, School of Dentistry and Dental Research Institute, Seoul National University, 101 Daehak-ro, Jongno-gu, Seoul 03080, Republic of Korea.
Department of Oral Medicine and Oral Diagnosis, School of Dentistry and Dental Research Institute, Seoul National University, 101 Daehak-ro, Jongno-gu, Seoul 03080, Republic of Korea; Institute on Aging, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea.
Arch Oral Biol. 2019 Jan;97:215-222. doi: 10.1016/j.archoralbio.2018.10.026. Epub 2018 Oct 24.
Diagnostic value of saliva depends on the reproducibility of data in repeatedly collected samples and predictable correlations between saliva and blood. We aimed to investigate the reliability, blood reflectance, and influence of blood contamination in the analysis of inflammatory and oxidative stress biomarkers in saliva samples.
In total, 37 healthy young male participants (26.7 ± 2.2 years) were included. Unstimulated whole saliva and blood samples were collected on the first visit, and saliva samples were collected again after 2-3 days. The concentrations of total protein and inflammatory [C-reactive protein (CRP), IL-1β, IL-6, and TNF-α] and oxidative stress [8-hydroxy-2'-deoxyguanosine (8-OHdG), malondialdehyde (MDA), and total antioxidant capacity (TAC)] biomarkers in saliva and blood, and as well as blood contamination biomarkers (transferrin and hemoglobin) in saliva were analyzed.
The intra-class correlations of all examined biomarkers except TNF-α were fair to excellent. Significant positive correlations between CRP and IL-6 and between total protein and TAC were stable in the saliva samples collected on different days. Notably, IL-6 was the only biomarker that showed a significant correlation between saliva and blood. As the concentration of salivary transferrin increased, the saliva/blood ratios of total protein and TAC also increased. The concentration of salivary hemoglobin did not affect the saliva/blood ratios of biomarkers.
The findings of this study are limited to healthy young males. For clinical applications, studies on salivary diagnostics should be performed for individual disease and health conditions, demographic characteristics, and biomarkers.
唾液的诊断价值取决于重复采集样本中数据的可重复性，以及唾液与血液之间可预测的相关性。本研究旨在探讨唾液中炎症和氧化应激生物标志物分析的可靠性、血液反射率以及血液污染的影响。
共纳入 37 名健康年轻男性参与者（26.7±2.2 岁）。在第一次就诊时采集未刺激全唾液和血液样本，然后在 2-3 天后再次采集唾液样本。分析唾液和血液中的总蛋白和炎症[C 反应蛋白（CRP）、白细胞介素-1β（IL-1β）、白细胞介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）]和氧化应激[8-羟基-2'-脱氧鸟苷（8-OHdG）、丙二醛（MDA）和总抗氧化能力（TAC）]生物标志物以及唾液中的血液污染生物标志物（转铁蛋白和血红蛋白）。
除 TNF-α外，所有检测生物标志物的组内相关系数均为中等到极好。CRP 和 IL-6 之间以及总蛋白和 TAC 之间的显著正相关在不同日期采集的唾液样本中稳定。值得注意的是，IL-6 是唯一显示与唾液和血液之间存在显著相关性的生物标志物。随着唾液中转铁蛋白浓度的增加，总蛋白和 TAC 的唾液/血液比值也随之增加。唾液中血红蛋白的浓度不影响生物标志物的唾液/血液比值。
本研究结果仅限于健康年轻男性。对于临床应用，应针对个体疾病和健康状况、人口统计学特征和生物标志物进行唾液诊断研究。
