Supercritical Extraction Department, New Chemical Syntheses Institute, Al. Tysiąclecia Państwa Polskiego 13A, 24-110 Puławy, Poland.
Import Export J.A. Szałas Company, Garbarska 125A, 26-600 Radom, Poland.
Molecules. 2018 Nov 3;23(11):2866. doi: 10.3390/molecules23112866.
Two different extraction methods were used for a comparative study of essential oils obtained from the scCO₂ (sc-supercritical) extract: microwave-assisted hydrodistillation (MAHD) and conventional hydrodistillation (HD). As a result, the best conditions for the maximum essential oil production were determined for the MAHD method at 335 W microwave power for 30 min at water to raw material ratio of 8:3. The obtained essential oils were enriched in β-myrcene in the amount of 74.13%⁻89.32% (wt%). Moreover, the first application for determination of the above-mentioned volatile compounds by supercritical fluid chromatography (SFC) with photo-diode array detection (PDA) is presented, which in comparison with gas chromatography coupled with mass spectrometry (GC-MS/MS) resulted in similar values for β-myrcene and α-humulene in obtained samples within less than 1 min.
采用两种不同的提取方法对超临界二氧化碳（sc-supercritical）提取物中的精油进行了比较研究：微波辅助水蒸馏（MAHD）和常规水蒸馏（HD）。结果表明，MAHD 法的最佳条件为微波功率 335 W，时间 30 min，水与原料的比例为 8:3，可获得最大的精油产量。所得精油中 β-月桂烯的含量为 74.13%⁻89.32%（wt%）。此外，首次提出了用超临界流体色谱（SFC）与光电二极管阵列检测（PDA）测定上述挥发性化合物的方法，与气相色谱-质谱联用（GC-MS/MS）相比，该方法在不到 1 分钟的时间内即可得到样品中β-月桂烯和α-葎草烯的相似值。
