Wang Yanhui, Zhang Huaqi, Teng Xiangyun, Guo Peiyu, Zuo Yuwei, Zhao Hui, Wang Peng, Liang Hui
Department of Nutrition and Food Hygiene School of Public Health Qingdao University Qingdao China.
Food Sci Nutr. 2022 Mar 29;10(8):2479-2495. doi: 10.1002/fsn3.2854. eCollection 2022 Aug.
Garlic oil (GO) is a kind of natural extract extracted from garlic, which has strong antioxidant activity. This study elucidates the protective mechanism of GO against alcohol-induced high triglyceride levels. Sixty male Sprague Dawley rats were assigned to five groups, including a control group (CON), a model group (MOD) treated with alcohol 56% v/v at 8 ml kg day for 2 weeks then 10 ml kg day for 8 weeks, a low-dose GO group (GO-L) given GO at 20 mg kg day, a high-dose GO group (GO-H) given GO at 40 mg kg day, and a positive group (POS) given diammonium glycyrrhizinate at 200 mg kg day. The results showed that GO could significantly reduce the serum and liver triglyceride levels caused by alcohol exposure ( < .05). The GO-H group significantly reduced MDA level, increased SOD and GSH-Px levels in serum, liver, and colon ( < .05), significantly increased the levels of Sirt1 and PGC-1α proteins and reduced FoxO1 protein level in liver ( < .05), and significantly increased the levels of ZO-1 and Claudin1 proteins in the colon compared to the MOD group ( < .05). The 16S rRNA sequencing showed that the intestinal flora of the GO-H group was significantly changed compared with the MOD group. In summary, GO has the potential to improve high triglyceride levels in serum and liver induced by alcohol exposure, which may be related to the inhibition of oxidative stress regulation of Sirt1 and its downstream proteins, and to the restoration of the intestinal barrier and intestinal flora.
大蒜油（GO）是从大蒜中提取的一种天然提取物，具有很强的抗氧化活性。本研究阐明了GO对酒精诱导的高甘油三酯水平的保护机制。将60只雄性Sprague Dawley大鼠分为五组，包括对照组（CON）、模型组（MOD），模型组以56% v/v的酒精按8 ml/kg·天处理2周，然后按10 ml/kg·天处理8周；低剂量GO组（GO-L），给予20 mg/kg·天的GO；高剂量GO组（GO-H），给予40 mg/kg·天的GO；阳性组（POS），给予200 mg/kg·天的甘草酸二铵。结果表明，GO可显著降低酒精暴露引起的血清和肝脏甘油三酯水平（P < 0.05）。GO-H组显著降低了血清、肝脏和结肠中的丙二醛（MDA）水平，提高了超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）水平（P < 0.05），显著提高了肝脏中沉默信息调节因子1（Sirt1）和过氧化物酶体增殖物激活受体γ共激活因子1α（PGC-1α）蛋白水平，降低了叉头框蛋白O1（FoxO1）蛋白水平（P < 0.05），与MOD组相比，结肠中闭合蛋白1（ZO-1）和Claudin1蛋白水平显著升高（P < 0.05）。16S核糖体RNA（rRNA）测序显示，与MOD组相比，GO-H组的肠道菌群发生了显著变化。综上所述，GO有可能改善酒精暴露诱导的血清和肝脏高甘油三酯水平，这可能与抑制Sirt1及其下游蛋白的氧化应激调节以及恢复肠道屏障和肠道菌群有关。
