Department of Orthopaedic Surgery, The First Affiliated Hospital of Xi'an Jiaotong University, No. 277 West Yanta Road, Yanta District, Xi'an, Shaanxi 710061, China.
Department of Hand Surgery, Xi'an Honghui Hospital, No. 555 Youyi East Road, Beilin District, Xi'an, Shaanxi 710054, China.
Mediators Inflamm. 2020 Apr 15;2020:8508906. doi: 10.1155/2020/8508906. eCollection 2020.
Diallyl disulfide (DADS) is the major organosulfur constituent in garlic, with a variety of pharmacological activities including antioxidant and anti-inflammatory. Here, we examined the potential antiedematous impact of DADS- versus carrageenan-mediated paw edema in mice. Carrageenan injection potentiated an inflammatory reaction as presented by the elevated serological C-reactive protein (CRP) levels and transcription of tumor necrosis factor-alpha (TNF-, Tnf), interleukin-1 beta (IL-1, Il1b), interleukin-2 (IL-2, Il2), inducible nitric oxide synthase (iNOS), nitric oxide (NO), cyclooxygenase-2 (COX-2, Ptgs2), prostaglandin E2 (PGE2), monocyte chemoattractant protein-1 (MCP-1, Ccl1), nuclear factor kappa B (NF-B), and myeloperoxidase (MPO) activity, while interleukin-10 (IL-10) was declined in the injured paw tissue. Additionally, carrageenan elevated lipid peroxidation in terms of malondialdehyde (MDA) and decreased glutathione content (GSH). Remarkably, DADS was found to inhibit the inflammatory signaling, suppressed the developed oxidative damage, and protected the histopathological alterations in the inflamed paw tissue in response to carrageenan injection. Our findings suggest that DADS could be used as an alternative therapy used to alleviate the pathophysiological changes associated with the genesis of paw edema through its potent anti-inflammatory and antioxidant impacts.
二烯丙基二硫（DADS）是大蒜中的主要有机硫成分，具有多种药理活性，包括抗氧化和抗炎作用。在这里，我们研究了 DADS 与角叉菜胶介导的小鼠爪肿胀的潜在抗水肿作用。角叉菜胶注射增强了炎症反应，表现为血清 C 反应蛋白（CRP）水平升高和肿瘤坏死因子-α（TNF-α，Tnf）、白细胞介素-1β（IL-1β，Il1b）、白细胞介素-2（IL-2，Il2）、诱导型一氧化氮合酶（iNOS）、一氧化氮（NO）、环氧化酶-2（COX-2，Ptgs2）、前列腺素 E2（PGE2）、单核细胞趋化蛋白-1（MCP-1，Ccl1）、核因子 kappa B（NF-B）和髓过氧化物酶（MPO）活性转录，而受伤爪组织中的白细胞介素-10（IL-10）下降。此外，角叉菜胶增加了丙二醛（MDA）水平的脂质过氧化和谷胱甘肽含量（GSH）的降低。值得注意的是，DADS 被发现可以抑制炎症信号转导，抑制已发生的氧化损伤，并保护炎症爪组织的组织病理学改变，以响应角叉菜胶注射。我们的研究结果表明，DADS 可作为一种替代疗法，通过其强大的抗炎和抗氧化作用，用于减轻与爪水肿发生相关的病理生理变化。
