中国鼠类和猪尾鼠携带腺相关病毒的分子流行病学调查。
A Molecular Epidemiological Investigation of Carriage of the Adeno-Associated Virus in Murine Rodents and House Shrews in China.
机构信息
Department of Epidemiology, School of Public Health, Guangdong Provincial Key Laboratory of Tropical Disease Research, Southern Medical University, Guangzhou, China.
Department of Epidemiology, Medical College of Hunan Normal University, Changsha, China.
出版信息
Intervirology. 2018;61(3):143-148. doi: 10.1159/000493799. Epub 2018 Nov 7.
OBJECTIVE
To investigate the prevalence of the adeno-associated virus (AAV) in murine rodents and house shrews in 4 provinces of China.
METHODS
A total of 469 murine rodents and 19 house shrews were captured between May 2015 and May 2017. Cap gene of AAV sequences was obtained to evaluate the genetic characteristics of rat AAV.
RESULTS
Rat AAVs were found in 54.7% (267/488) of throat swabs, 14.3% (70/488) of fecal samples, and 18.4% (41/223) of serum samples. Rat AAVs were detected in 3 species of murine rodents including Rattus norvegicus (34.8%), R. tanezumi (43.0%), and R. losea (2.3%), and house shrews (Suncus murinus) (26.1%) from the selected sampling sites. Fourteen near-full-length Cap gene sequences, ranging in length from 2,156 to 2,169 nt, were isolated from the fecal samples of R. norvegicus and R. tanezumi. These 14 sequences shared a high identity of 97.4% at the nucleotide level and 99.1% at the amino acid level. Phylogenetic analysis showed that the rat AAV formed a distinct clade, distinguishable from the AAV discovered in humans and in other animals.
CONCLUSIONS
A high prevalence of rat AAV that was highly conserved within the Cap gene was found in 3 common murine rodents and house shrews in China.
目的
调查中国 4 个省份的鼠类和猪尾鼠中腺相关病毒（AAV）的流行情况。
方法
2015 年 5 月至 2017 年 5 月期间共捕获 469 只鼠类和 19 只猪尾鼠。获得 AAV 的 Cap 基因序列，以评估大鼠 AAV 的遗传特征。
结果
在 488 个咽喉拭子、14.3%（70/488）的粪便样本和 18.4%（41/223）的血清样本中发现了大鼠 AAV。在选定采样点的 3 种鼠类（包括挪威鼠（Rattus norvegicus）、田鼠（R. tanezumi）和黑线姬鼠（R. losea））和猪尾鼠（Suncus murinus）中检测到大鼠 AAV。从挪威鼠和田鼠的粪便样本中分离出 14 个全长 Cap 基因序列，长度从 2156 到 2169 个核苷酸。这 14 个序列在核苷酸水平上的同源性高达 97.4%，在氨基酸水平上的同源性高达 99.1%。系统发育分析表明，大鼠 AAV 形成了一个独特的分支，与在人类和其他动物中发现的 AAV 不同。
结论
在中国 3 种常见的鼠类和猪尾鼠中发现了具有高度保守性的 Cap 基因的大鼠 AAV 高流行率。
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