Ahmed Noorsher, Sukovich David, Abate Adam R
Department of Bioengineering and Therapeutic Sciences, California Institute of Quantitative Biosciences (QB3), University of California, San Francisco, CA 94115, USA.
Micromachines (Basel). 2016 Sep 6;7(9):161. doi: 10.3390/mi7090161.
Microfluidic devices are valuable for a variety of biotechnology applications, such as synthesizing biochemical libraries, screening enzymes, and analyzing single cells. However, normally, the devices are controlled using specialized pumps, which require expert knowledge to operate. Here, we demonstrate operation of poly(dimethylsiloxane) devices without pumps. We build a scaffold that holds the device and reagents to be infused in a format that can be inserted into a 50 mL falcon tube and spun in a common lab centrifuge. By controlling the device design and centrifuge spin speed, we infuse the reagents at controlled flow rates. We demonstrate the encapsulation and culture of clonal colonies of red and green in droplets seeded from single cells.
微流控设备在各种生物技术应用中都很有价值，比如合成生化文库、筛选酶以及分析单细胞。然而，通常这些设备是通过专用泵来控制的，而操作这些泵需要专业知识。在此，我们展示了无需泵的聚二甲基硅氧烷设备的操作。我们构建了一个支架，将设备和待注入的试剂以一种可以插入50毫升离心管并在普通实验室离心机中离心的形式固定住。通过控制设备设计和离心机转速，我们以可控的流速注入试剂。我们展示了从单细胞接种的液滴中对红色和绿色克隆菌落的封装和培养。
