Guo Lei, He Yuan, Li Heng, Chen Yong, Zhu Fanli, Yang Mengli, Yang Chengyun, Dai Qing, Shi Haijing, Liu Longding
Department of respiratory infection, Kunming National High-level Biosafety Research Center, Institute of Medical Biology, Chinese Academy of Medical Science, Kunming, Yunnan 650118, China.
Department of respiratory infection, Kunming National High-level Biosafety Research Center, Institute of Medical Biology, Chinese Academy of Medical Science, Kunming, Yunnan 650118, China
Biol Open. 2018 Dec 10;7(12):bio035006. doi: 10.1242/bio.035006.
High-level biosafety laboratories (BSL), such as BSL-3 and BSL-4, which deal with high infectivity and virulence pathogens, have become indispensable. Mice are frequently used in animal BSL (ABSL) to establish animal models for infection and to evaluate immune responses. A project of monitoring and evaluation on the physiology and immune status of mice housed in different ABSL labs was performed in the ABSL-2/3/4 labs of Kunming National High-level Biosafety Research Center, China. Female Kunming mice were housed in the ABSL-2/3/4 labs for 1 month, and mouse behavior, body physiology/immune status, pulmonary immune status and respiratory bacteria composition were evaluated and compared among mice from the different labs. Mice settled in their new housing environment of the different labs after transfer and gained weight steadily. Blood hematology testing, serum cytokine/chemokine profiles and blood/spleen lymphocyte constitutions were comparable between the ABSL-2/3/4 labs. The numbers of different pulmonary leukocytes in the bronchoalveolar lavage fluid were at baseline levels in mice from the ABSL-2/3/4 labs. Diversity and dominance of mice respiratory bacteria were semblable among the ABSL-2/3/4 labs. Our results confirm the stability of physiology and immune status of Kunming mice maintained in different ABSL-2/3/4 labs for at least 1 month.
诸如生物安全3级（BSL-3）和生物安全4级（BSL-4）等处理高传染性和高致病性病原体的高级生物安全实验室已变得不可或缺。小鼠常用于动物生物安全实验室（ABSL），以建立感染动物模型并评估免疫反应。在中国昆明国家高级生物安全研究中心的ABSL-2/3/4实验室开展了一项关于不同ABSL实验室中饲养小鼠的生理和免疫状态的监测与评估项目。将雌性昆明小鼠置于ABSL-2/3/4实验室中饲养1个月，对来自不同实验室的小鼠的行为、身体生理/免疫状态、肺部免疫状态及呼吸道细菌组成进行评估和比较。转移后，小鼠在不同实验室的新饲养环境中适应下来并体重稳步增加。ABSL-2/3/4实验室之间的血液血液学检测、血清细胞因子/趋化因子谱及血液/脾脏淋巴细胞组成具有可比性。ABSL-2/3/4实验室小鼠支气管肺泡灌洗液中不同肺白细胞的数量处于基线水平。ABSL-2/3/4实验室之间小鼠呼吸道细菌的多样性和优势度相似。我们的结果证实了在不同ABSL-2/3/4实验室中饲养至少1个月的昆明小鼠的生理和免疫状态的稳定性。
