Henneman John R, McQuade Elizabeth A, Sullivan Rachael R, Downard Jen, Thackrah Ashley, Hislop Meaghan
Biosecurity Research Institute, Kansas State University, Manhattan, Kansas, USA.
Department of Science and Research, CURIS System, Oviedo, Florida, USA.
Appl Biosaf. 2022 Mar 1;27(1):7-14. doi: 10.1089/apb.2021.0012. Epub 2022 Mar 15.
The applications of fumigation and the challenges that high-containment facilities face in achieving effective large volume decontamination are well understood. The Biosecurity Research Institute at Kansas State University sought to evaluate a novel system within their biosafety level 3 (BSL-3) and animal biosafety level 3 agriculture (ABSL-3Ag) facility.
The system chosen for this study is the CURIS Hybrid Hydrogen Peroxide (HHP) system, comprising a mobile 36-pound (16 kg) device delivering a proprietary 7% hydrogen peroxide (HO) solution. To examine the system's efficacy in multiple laboratory settings, two BSL-3 laboratories (2,281 [65 m] and 4,668 ft [132 m]) with dropped ceiling interstitial spaces and an ABSL-3Ag necropsy suite (44,212 ft [1,252 m]) with 21-foot (6.4 m) ceilings were selected. Biological indicators (BIs) of (1.7 × 10 organisms) on steel spore carriers and HO chemical indicators (CIs) were used to provide validation.
After cycle optimization, the smaller laboratory had a total of 60 BIs over two treatments that demonstrated a greater than 6-log reduction of bacterial spores. The larger laboratory (192 BIs) and the necropsy suite (206 BIs) had no BIs positive for spore growth when incubated at 60°C for 24 h per manufacturer's specifications.
Overall successful results through multiple components of this study demonstrate that the HHP device, paired with the pulsed 7% HO solution, achieved efficacy regardless of variables in laboratory size and layout. Perceived challenges such as 21-ft (6.4 m) ceiling heights, active equipment, and difficult to access ceiling interstitial spaces proved unfounded. Given the successful sterilization of all challenged BIs, the HHP system presents a useful alternative for high level decontamination within BSL-3 and ABSL-3Ag facilities.
熏蒸的应用以及高防护设施在实现有效大体积去污方面面临的挑战已广为人知。堪萨斯州立大学的生物安全研究所试图在其生物安全三级（BSL-3）和动物生物安全三级农业（ABSL-3Ag）设施中评估一种新型系统。
本研究选择的系统是CURIS混合过氧化氢（HHP）系统，它由一个移动的36磅（16千克）设备组成，该设备可输送一种专利的7%过氧化氢（HO）溶液。为了检验该系统在多种实验室环境中的功效，选择了两个带有吊顶夹层空间的BSL-3实验室（2281平方英尺[65平方米]和4668平方英尺[132平方米]）以及一个天花板高21英尺（6.4米）的ABSL-3Ag剖检室（44212平方英尺[1252平方米]）。使用钢质孢子载体上的（1.7×10个生物体）生物指示剂（BIs）和HO化学指示剂（CIs）进行验证。
经过循环优化后，较小的实验室在两次处理中共有60个BIs，显示出细菌孢子减少了6个对数级以上。按照制造商的规格，在60°C下孵育24小时后，较大的实验室（192个BIs）和剖检室（206个BIs）的孢子生长均无BIs呈阳性。
通过本研究的多个组成部分总体取得的成功结果表明，HHP设备与脉冲式7%HO溶液配合使用，无论实验室规模和布局的变量如何，都能实现有效去污。诸如21英尺（6.4米）的天花板高度、有源设备以及难以进入的天花板夹层空间等预期挑战被证明是没有根据的。鉴于所有受挑战的BIs均成功灭菌，HHP系统为BSL-3和ABSL-3Ag设施内的高水平去污提供了一种有用的替代方案。
