Liu Dong, Yang Yang, An Shaoshan, Wang Honglei, Wang Ying
State Key Laboratory of Soil Erosion and Dryland Farming on the Loess Plateau, Northwest A&F University, Yangling, China.
Kunming Institute of Botany, Chinese Academy of Sciences, Kunming, China.
Front Microbiol. 2018 Oct 18;9:2456. doi: 10.3389/fmicb.2018.02456. eCollection 2018.
The rigorous environmental stress of the severely eroded Loess Plateau may have promoted specific soil bacterial communities in comparison to other eco-environmental regions. In order to unmask the bacterial diversity and most influential environmental parameters, Illumina MiSeq high throughput sequencing of 16S rRNA from 24 representative soil samples collected across south-east to north-west transect of the Loess Plateau in northern Shaanxi, China was conducted. This high-throughput sequencing revealed a total of 1,411,001 high quality sequences that classified into 38 phyla, 127 classes, >240 orders, and over 650 genera, suggesting a high bacterial richness across the Loess Plateau soils. The seven dominant groups were: , and (relative abundance of >5%). Increasing/decreasing soil pH and geographic longitudinal distance correlated significantly with increasing/decreasing bacterial richness and diversity indices. Pairwise correlation analysis showed higher bacterial diversity at longitudinal gradients across 107°39'-109°15' (south-east to north-west) in our studied Chinese loess zone. Variation partitioning analysis indicated significant influence of soil characteristics (~40.4%) than geographical distance (at a landscape scale of ~400 km) that was responsible for 13.6% of variation in bacterial community structure from these soils. Overall, contemporary soil characteristics structure the bacterial community in Loess Plateau soil to a greater extent than the spatial distances along the loess transect.
与其他生态环境区域相比，严重侵蚀的黄土高原严峻的环境压力可能促进了特定土壤细菌群落的形成。为了揭示细菌多样性和最具影响力的环境参数，对采自中国陕北黄土高原从东南到西北样带的24个代表性土壤样本进行了16S rRNA的Illumina MiSeq高通量测序。这种高通量测序共揭示了1,411,001条高质量序列，这些序列分为38个门、127个纲、>240个目和650多个属，表明黄土高原土壤中细菌丰富度很高。七个优势菌群为： ， ， ， ， ， ， （相对丰度>5%）。土壤pH值的升高/降低和地理经度距离的增加/减少与细菌丰富度和多样性指数的增加/减少显著相关。成对相关性分析表明，在我们研究的中国黄土带107°39′-109°15′（东南到西北）的纵向梯度上细菌多样性更高。变异分割分析表明，土壤特征（约40.4%）比地理距离（在约400公里的景观尺度上）对这些土壤细菌群落结构变异的影响更大，地理距离占13.6%。总体而言，与沿黄土样带的空间距离相比，当代土壤特征在更大程度上构建了黄土高原土壤中的细菌群落。
