Yang Yuankun, Yang Xiufen, Dong Yijie, Qiu Dewen
The State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, China.
Front Microbiol. 2018 Oct 23;9:2535. doi: 10.3389/fmicb.2018.02535. eCollection 2018.
is one of the most notorious pathogenic species that causes serious plant diseases and substantial losses in agriculture throughout the world. We identified BcXyl1 from that exhibited xylanase activity. Expression of the gene was strongly induced in infecting and tomato plants, and deletion strains severely compromised the virulence of . BcXyl1 induced strong cell death in several plants, and cell death activity of BcXyl1 was independent of its xylanase activity. Purified BcXyl1 triggered typically PAMP-triggered immunity (PTI) responses and conferred resistance to and TMV in tobacco and tomato plants. A 26-amino acid peptide of BcXyl1 was sufficient for elicitor function. Furthermore, the BcXyl1 death-inducing signal was mediated by the plant LRR receptor-like kinases (RLKs) BAK1 and SOBIR1. Our data suggested that BcXyl1 contributed to virulence and induced plant defense responses.
是最臭名昭著的致病物种之一，会引发严重的植物疾病，并在全球农业中造成重大损失。我们从 中鉴定出了具有木聚糖酶活性的BcXyl1。该 基因的表达在感染 和番茄植株时被强烈诱导，并且 缺失菌株严重损害了 的毒力。BcXyl1在几种植物中诱导强烈的细胞死亡，且BcXyl1的细胞死亡活性与其木聚糖酶活性无关。纯化的BcXyl1引发典型的病原体相关分子模式触发的免疫（PTI）反应，并赋予烟草和番茄植株对 和烟草花叶病毒（TMV）的抗性。BcXyl1的一个26个氨基酸的肽段足以发挥激发子功能。此外，BcXyl1诱导细胞死亡的信号由植物富含亮氨酸重复序列的类受体激酶（RLK）BAK1和SOBIR1介导。我们的数据表明，BcXyl1有助于 的毒力并诱导植物防御反应。
