1 Laboratory of Cotton Disease, Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences, Beijing, 100193, China; and.
2 Department of Plant Pathology, University of California, Davis, U.S.A.
Mol Plant Microbe Interact. 2018 Feb;31(2):260-273. doi: 10.1094/MPMI-06-17-0136-R. Epub 2017 Dec 20.
Cutinases have been implicated as important enzymes during the process of fungal infection of aerial plant organs. The function of cutinases in the disease cycle of fungal pathogens that invade plants through the roots has been less studied. Here, functional analysis of 13 cutinase (carbohydrate esterase family 5 domain-containing) genes (VdCUTs) in the highly virulent vascular wilt pathogen Verticillium dahliae Vd991 was performed. Significant sequence divergence in cutinase family members was observed in the genome of V. dahliae Vd991. Functional analyses demonstrated that only VdCUT11, as purified protein, induced cell death and triggered defense responses in Nicotiana benthamiana, cotton, and tomato plants. Virus-induced gene silencing showed that VdCUT11 induces plant defense responses in Nicotiana benthamania in a BAK1 and SOBIR-dependent manner. Furthermore, coinfiltration assays revealed that the carbohydrate-binding module family 1 protein (VdCBM1) suppressed VdCUT11-induced cell death and other defense responses in N. benthamiana. Targeted deletion of VdCUT11 in V. dahliae significantly compromised virulence on cotton plants. The cutinase VdCUT11 is an important secreted enzyme and virulence factor that elicits plant defense responses in the absence of VdCBM1.
角质酶在真菌对空气植物器官的感染过程中被认为是重要的酶。角质酶在通过根部侵入植物的真菌病原体的疾病循环中的功能研究较少。在这里，对高度毒力的维管束萎蔫病原菌Verticillium dahliae Vd991 中的 13 个角质酶（碳水化合物酯酶家族 5 结构域）基因（VdCUTs）进行了功能分析。在 V. dahliae Vd991 的基因组中观察到角质酶家族成员的显著序列差异。功能分析表明，只有 VdCUT11 作为纯化蛋白，在 Nicotiana benthamiana、棉花和番茄植物中诱导细胞死亡并引发防御反应。病毒诱导的基因沉默表明，VdCUT11 在 Nicotiana benthamania 中以 BAK1 和 SOBIR 依赖的方式诱导植物防御反应。此外，共浸润测定表明，碳水化合物结合模块家族 1 蛋白（VdCBM1）抑制了 VdCUT11 在 N. benthamiana 中诱导的细胞死亡和其他防御反应。在 V. dahliae 中靶向缺失 VdCUT11 显著削弱了其对棉花植物的毒力。角质酶 VdCUT11 是一种重要的分泌酶和毒力因子，在缺乏 VdCBM1 的情况下会引发植物防御反应。
