Loeffelholz M J, Modrzakowski M C
Infect Immun. 1987 Sep;55(9):2296-9. doi: 10.1128/iai.55.9.2296-2299.1987.
Growth of Acinetobacter calcoaceticus on specific alkanes altered the outer membrane permeability of the organism, as indicated by a change in sensitivity to the antibiotic actinomycin D. As the carbon length of the alkane energy source decreased, outer membrane permeability and susceptibility to actinomycin D increased. Concomitant with the increase in outer membrane permeability, A. calcoaceticus became more susceptible to the oxygen-independent antimicrobial activity of extracted contents from rat polymorphonuclear leukocyte granules. Individual fractions of granule extract possessed no antimicrobial activity against A. calcoaceticus. The alkane-induced change in outer membrane permeability was not associated with alterations of lipopolysaccharide O antigen. An outer membrane permeability mechanism, independent of changes in lipopolysaccharide content, mediating susceptibility to the oxygen-independent antimicrobial activity of rat polymorphonuclear leukocyte granule contents is suggested.
乙酸钙不动杆菌在特定烷烃上的生长改变了该生物体的外膜通透性，这表现为对抗生素放线菌素D的敏感性变化。随着烷烃能源碳链长度的缩短，外膜通透性和对放线菌素D的敏感性增加。伴随着外膜通透性的增加，乙酸钙不动杆菌对从大鼠多形核白细胞颗粒中提取的内容物的非氧依赖性抗菌活性变得更加敏感。颗粒提取物的各个组分对乙酸钙不动杆菌没有抗菌活性。烷烃诱导的外膜通透性变化与脂多糖O抗原的改变无关。提示存在一种独立于脂多糖含量变化的外膜通透性机制，介导对大鼠多形核白细胞颗粒内容物非氧依赖性抗菌活性的敏感性。
