Modrzakowski M C, Cooney M H, Martin L E, Spitznagel J K
Infect Immun. 1979 Mar;23(3):587-91. doi: 10.1128/iai.23.3.587-591.1979.
Proteins from human polymorphonuclear leukocyte granules were extracted with 0.2 M acetate, pH 4.0, and fractionated by Sephadex G-100 column chromatography. The fractions demonstrated selective bactericidal action against a deep rough cell wall mutant of Escherichia coli O111:B4 with rough lipopolysacharide and cell wall mutants of Salmonella typhimurium LT-2 with lipoplysacharide of Ra, Rc, Rd1, Rd2, and Re types. Smooth parent strains were most resistant to the bactericidal action. Fractions with greatest activity for the mutants were from valley regions (regions of low protein concentration) between three high protein peaks comprising myeloperoxidase, protease, and lysozyme, respectively. Susceptibility of the mutants to bactericidal action increased as sugar residues decreased in lipopolysaccharide. Gram-positive bacteria were susceptible to different fractions than were the gram-negative bacteria.
用人多形核白细胞颗粒中的蛋白质，在pH 4.0的0.2M醋酸盐中进行提取，并用葡聚糖G - 100柱色谱法进行分级分离。这些级分对具有粗糙脂多糖的大肠杆菌O111:B4深粗糙细胞壁突变体以及具有Ra、Rc、Rd1、Rd2和Re型脂多糖的鼠伤寒沙门氏菌LT - 2细胞壁突变体表现出选择性杀菌作用。光滑的亲本菌株对杀菌作用最具抗性。对突变体活性最高的级分来自分别包含髓过氧化物酶、蛋白酶和溶菌酶的三个高蛋白峰之间的低谷区域（低蛋白浓度区域）。随着脂多糖中糖残基减少，突变体对杀菌作用的敏感性增加。革兰氏阳性菌与革兰氏阴性菌对不同的级分敏感。
