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基于宏基因组学的中国云南省蚊虫病毒组分析揭示了登革热病毒和 Zika 病毒基因。

Metagenomic Analysis of in Mosquito Viromes Isolated From Yunnan Province in China Reveals Genes From Dengue and Zika Viruses.

机构信息

Yanbian University Medical College, Yanji, China.

Institute of Military Veterinary, Academy of Military Medical Sciences, Changchun, China.

出版信息

Front Cell Infect Microbiol. 2018 Oct 24;8:359. doi: 10.3389/fcimb.2018.00359. eCollection 2018.

DOI:10.3389/fcimb.2018.00359
PMID:30406038
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6207848/
Abstract

More than 6,000 mosquitoes of six species from six sites were collected and tested for their virome using metagenomics sequencing and bioinformatic analysis. The identified viral sequences belonged to more than 50 viral families. The results were verified by PCR of selected viruses in all mosquitoes, followed by phylogenetic analysis. In the present study, we identified the partial dengue virus (DENV), Zika virus (ZIKV), and Japanese encephalitis virus (JEV) sequences in mosquitoes. Metagenomic analysis and the PCR amplification revealed three DENV sequences, one of which encodes a partial envelope protein. Two ZIKV sequences both encoding partial nonstructural protein 3 and one JEV sequence encoding the complete envelope protein were identified. There was variability in the viral titers of the newly isolated virus JEV-China/YN2016-1 of different passage viruses. The newly identified Zika virus gene from ZIKV-China/YN2016-1 was an Asian genotype and shared the highest nucleotide sequence identity (97.1%) with a ZIKV sequence from Thailand isolated in 2004. Phylogenetic analysis of ZIKV-China/YN2016-1 and ZIKV-China/YN2016-2 with known genes indicated that ZIKV has propagated in Yunnan province, China.

摘要

从六个地点收集了超过 6000 只六种蚊子，并通过宏基因组测序和生物信息学分析对其病毒组进行了测试。鉴定出的病毒序列属于 50 多种病毒家族。通过对所有蚊子中选定病毒的 PCR 进行验证，然后进行系统发育分析，对结果进行了验证。在本研究中，我们在蚊子中鉴定出了部分登革热病毒（DENV）、寨卡病毒（ZIKV）和日本脑炎病毒（JEV）序列。宏基因组分析和 PCR 扩增显示了三个 DENV 序列，其中一个编码部分包膜蛋白。鉴定出两个 ZIKV 序列均编码部分非结构蛋白 3，一个 JEV 序列编码完整包膜蛋白。不同传代病毒的新分离病毒 JEV-China/YN2016-1 的病毒滴度存在差异。从 ZIKV-China/YN2016-1 中鉴定出的新寨卡病毒基因是亚洲基因型，与 2004 年在泰国分离的寨卡病毒序列的核苷酸序列同一性最高（97.1%）。与已知基因的 ZIKV-China/YN2016-1 和 ZIKV-China/YN2016-2 的系统发育分析表明，寨卡病毒在中国云南省传播。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c3e/6207848/d75407b8ca8c/fcimb-08-00359-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c3e/6207848/be68268829ce/fcimb-08-00359-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c3e/6207848/22b4efd26f0f/fcimb-08-00359-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c3e/6207848/38af31942998/fcimb-08-00359-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c3e/6207848/2323dd6759be/fcimb-08-00359-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c3e/6207848/bcac2e84434f/fcimb-08-00359-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c3e/6207848/d75407b8ca8c/fcimb-08-00359-g0006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c3e/6207848/be68268829ce/fcimb-08-00359-g0001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c3e/6207848/22b4efd26f0f/fcimb-08-00359-g0002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c3e/6207848/38af31942998/fcimb-08-00359-g0003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c3e/6207848/2323dd6759be/fcimb-08-00359-g0004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c3e/6207848/bcac2e84434f/fcimb-08-00359-g0005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8c3e/6207848/d75407b8ca8c/fcimb-08-00359-g0006.jpg

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