Frey Kenneth G, Biser Tara, Hamilton Theron, Santos Carlos J, Pimentel Guillermo, Mokashi Vishwesh P, Bishop-Lilly Kimberly A
Naval Medical Research Center - Frederick, Fort Detrick, MD, USA.; Henry M. Jackson Foundation, Bethesda, MD, USA.
Naval Medical Research Center - Frederick, Fort Detrick, MD, USA.; Hood College, Frederick, MD, USA.
Evol Bioinform Online. 2016 Jun 16;12(Suppl 2):1-12. doi: 10.4137/EBO.S38518. eCollection 2016.
Mosquitoes are efficient, militarily relevant vectors of infectious disease pathogens, including many RNA viruses. The vast majority of all viruses are thought to be undiscovered. Accordingly, recent studies have shown that viruses discovered in insects are very divergent from known pathogens and that many of them lack appropriate reference sequences in the public databases. Given that the majority of viruses are likely still undiscovered, environ mental sampling stands to provide much needed reference samples as well as genetic sequences for comparison. In this study, we sought to determine whether samples of mosquitoes collected from different sites (the Caribbean and locations on the US East Coast) could be differentiated using metagenomic analysis of the RNA viral fraction. We report here distinct virome profiles, even from samples collected short distances apart. In addition to profiling the previously known viruses from these samples, we detected a number of viruses that have been previously undiscovered.
蚊子是传染病病原体（包括许多RNA病毒）的高效、与军事相关的传播媒介。据认为，所有病毒中的绝大多数尚未被发现。因此，最近的研究表明，在昆虫中发现的病毒与已知病原体差异很大，而且其中许多在公共数据库中缺乏合适的参考序列。鉴于大多数病毒可能仍未被发现，环境采样有望提供急需的参考样本以及用于比较的基因序列。在本研究中，我们试图确定从不同地点（加勒比地区和美国东海岸的地点）收集的蚊子样本是否可以通过对RNA病毒部分的宏基因组分析来区分。我们在此报告，即使是从相距很短距离采集的样本，其病毒组特征也截然不同。除了分析这些样本中先前已知的病毒外，我们还检测到了一些先前未被发现的病毒。
