Jung Jaehoon, Nakajima Masahiro, Takeuchi Masaru, Najdovski Zoran, Huang Qiang, Fukuda Toshio
Department of Micro-Nano Systems Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan.
Medical Device Development Center, Daegu-Gyeongbuk Medical Innovation Foundation (DGMIF), 80 Cheombok-Ro, Dong-gu, Daegu 41061, Korea.
Micromachines (Basel). 2016 Mar 19;7(3):50. doi: 10.3390/mi7030050.
This work presents a novel method to assess the condition of () through a resistance measurement of its undulatory locomotion speed inside a micro channel. As the worm moves over the electrode inside the micro channel, the length of the electrode changes, consequently behaving like a strain gauge. In this paper, the electrotaxis was applied for controlling the direction of motion of as an external stimulus, resulting in the worm moving towards the cathode of the circuit. To confirm the proposed measurement method, a microfluidic device was developed that employs a sinusoidal channel and a thin polydimethylsiloxane (PDMS) layer with an electrode. The PDMS layer maintains a porous structure to enable the flexibility of the electrode. In this study, 6 measurements were performed to obtain the speed of an early adult stage , where the measured average speed was 0.35 (±0.05) mm/s. The results of this work demonstrate the application of our method to measure the speed of undulatory locomotion. This novel approach can be applied to make such measurements without an imaging system, and more importantly, allows directly to detect the locomotion of using an electrical signal (, the change in resistance).
这项工作提出了一种新颖的方法，通过测量其在微通道内波动运动速度的电阻来评估（）的状况。当蠕虫在微通道内的电极上移动时，电极的长度会发生变化，因此其表现类似于应变仪。在本文中，电趋性被用作外部刺激来控制（）的运动方向，使蠕虫向电路的阴极移动。为了验证所提出的测量方法，开发了一种微流体装置，该装置采用正弦形通道和带有电极的薄聚二甲基硅氧烷（PDMS）层。PDMS层保持多孔结构以确保电极的柔韧性。在本研究中，进行了6次测量以获得成年早期（）的速度，测得的平均速度为0.35（±0.05）mm/s。这项工作的结果证明了我们的方法在测量（）波动运动速度方面的应用。这种新颖的方法可以在没有成像系统的情况下进行此类测量，更重要的是，能够直接利用电信号（即电阻变化）检测（）的运动。
