Zhang Yushan, Watts Benjamin R, Guo Tianyi, Zhang Zhiyi, Xu Changqing, Fang Qiyin
School of Biomedical Engineering, McMaster University, 1280 Main Street West, Hamilton, ON L8S 4L8, Canada.
ArtIC Photonics, 260 Terence Matthews Cres, Ottawa, ON K2M 2C7, Canada.
Micromachines (Basel). 2016 Apr 15;7(4):70. doi: 10.3390/mi7040070.
Optofluidic devices combining micro-optical and microfluidic components bring a host of new advantages to conventional microfluidic devices. Aspects, such as optical beam shaping, can be integrated on-chip and provide high-sensitivity and built-in optical alignment. Optofluidic microflow cytometers have been demonstrated in applications, such as point-of-care diagnostics, cellular immunophenotyping, rare cell analysis, genomics and analytical chemistry. Flow control, light guiding and collecting, data collection and data analysis are the four main techniques attributed to the performance of the optofluidic microflow cytometer. Each of the four areas is discussed in detail to show the basic principles and recent developments. 3D microfabrication techniques are discussed in their use to make these novel microfluidic devices, and the integration of the whole system takes advantage of the miniaturization of each sub-system. The combination of these different techniques is a spur to the development of microflow cytometers, and results show the performance of many types of microflow cytometers developed recently.
结合微光学和微流体组件的光流控设备为传统微流体设备带来了许多新优势。诸如光束整形等方面可以集成在芯片上，并提供高灵敏度和内置光学对准。光流控微流控细胞仪已在即时诊断、细胞免疫表型分析、稀有细胞分析、基因组学和分析化学等应用中得到验证。流量控制、光导与收集、数据采集和数据分析是光流控微流控细胞仪性能的四项主要技术。对这四个领域分别进行详细讨论，以展示其基本原理和最新进展。讨论了3D微加工技术在制造这些新型微流体设备中的应用，整个系统的集成利用了每个子系统的小型化优势。这些不同技术的结合推动了微流控细胞仪的发展，结果展示了最近开发的多种类型微流控细胞仪的性能。
