Ateya Daniel A, Erickson Jeffrey S, Howell Peter B, Hilliard Lisa R, Golden Joel P, Ligler Frances S
Center for Bio/Molecular Science and Engineering, Naval Research Laboratory, Washington, DC, 20375, USA.
Anal Bioanal Chem. 2008 Jul;391(5):1485-98. doi: 10.1007/s00216-007-1827-5. Epub 2008 Jan 29.
Recent developments in microflow cytometry have concentrated on advancing technology in four main areas: (1) focusing the particles to be analyzed in the microfluidic channel, (2) miniaturization of the fluid-handling components, (3) miniaturization of the optics, and (4) integration and applications development. Strategies for focusing particles in a narrow path as they pass through the detection region include the use of focusing fluids, nozzles, and dielectrophoresis. Strategies for optics range from the use of microscope objectives to polymer waveguides or optical fibers embedded on-chip. While most investigators use off-chip fluidic control, there are a few examples of integrated valves and pumps. To date, demonstrations of applications are primarily used to establish that the microflow systems provide data of the same quality as laboratory systems, but new capabilities-such as automated sample staining-are beginning to emerge. Each of these four areas is discussed in detail in terms of the progress of development, the continuing limitations, and potential future directions for microflow cytometers.
（1）在微流控通道中对待分析颗粒进行聚焦；（2）流体处理组件的小型化；（3）光学器件的小型化；（4）集成与应用开发。当颗粒通过检测区域时，将其聚焦在狭窄路径上的策略包括使用聚焦流体、喷嘴和介电泳。光学方面的策略涵盖从使用显微镜物镜到嵌入芯片的聚合物波导或光纤。虽然大多数研究人员采用芯片外流体控制，但也有一些集成阀门和泵的实例。迄今为止，应用演示主要用于证明微流控系统能够提供与实验室系统质量相当的数据，不过诸如自动样品染色等新功能也已开始出现。本文将详细讨论这四个领域中微流控细胞仪的发展进程、持续存在的局限性以及潜在的未来发展方向。
