Hawker J R, McHenry C S
J Biol Chem. 1987 Sep 15;262(26):12722-7.
We have established two murine hybridoma cell lines that secrete monoclonal antibodies directed against the tau subunit of the DNA polymerase III holoenzyme of Escherichia coli. Both antibodies have been purified and identified to be of the IgG1 class. Competition assays indicate that they bind to two distinct portions of the tau subunit. These antibodies have been used to demonstrate that tau is an integral part of all DNA polymerase III holoenzyme assemblies and that tau is the product of the dnaZX gene. Both of the antibodies react only with tau, not with gamma, the other protein product of the dnaZX gene. Immunoprecipitation studies demonstrated that tau is contained within the same enzyme assemblies as gamma (dnaZ protein). This observation is discussed in the light of the DNA polymerase III holoenzyme functioning as an asymmetric dimer, capable of coordinating leading with lagging strand replication.
我们建立了两种小鼠杂交瘤细胞系，它们分泌针对大肠杆菌DNA聚合酶III全酶的tau亚基的单克隆抗体。两种抗体均已纯化，并鉴定为IgG1类。竞争试验表明它们结合到tau亚基的两个不同部分。这些抗体已被用于证明tau是所有DNA聚合酶III全酶组装体的一个组成部分，并且tau是dnaZX基因的产物。两种抗体仅与tau反应，而不与dnaZX基因的另一种蛋白质产物gamma反应。免疫沉淀研究表明，tau与gamma（dnaZ蛋白）包含在相同的酶组装体中。根据DNA聚合酶III全酶作为不对称二聚体发挥作用，能够协调前导链与滞后链复制的情况对这一观察结果进行了讨论。
