Carter J R, Franden M A, Aebersold R, Kim D R, McHenry C S
University of Colorado Health Sciences Center, Department of Biochemistry, Biophysics and Genetics, Denver 80262.
Nucleic Acids Res. 1993 Jul 11;21(14):3281-6. doi: 10.1093/nar/21.14.3281.
The gene encoding the theta subunit of DNA polymerase III holoenzyme, designated holE, was isolated using a strategy in which peptide sequence was used to derive a DNA hybridization probe. Sequencing of the gene, which maps to 41.43 centisomes of the chromosome, revealed a 76-codon open reading frame predicted to produce a protein of 8,846 Da. When placed in a tac promoter expression vector, the open reading frame directed expression of a protein, that comigrated with authentic theta subunit from purified holoenzyme, to 6% of total soluble protein.
编码DNA聚合酶III全酶θ亚基的基因（命名为holE）是通过一种利用肽序列推导DNA杂交探针的策略分离得到的。该基因定位于染色体的41.43厘摩处，对其测序发现一个76密码子的开放阅读框，预计可产生一个8846道尔顿的蛋白质。当置于tac启动子表达载体中时，该开放阅读框指导合成一种蛋白质，它与纯化的全酶中的真实θ亚基迁移情况相同，占总可溶性蛋白的6%。
