Paek Jayoung, Shin Yeseul, Kook Joong-Ki, Chang Young-Hyo
1ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
2Korean Collection for Oral Microbiology and Department of Oral Biochemistry, School of Dentistry, Chosun University, Gwangju 501-759, Republic of Korea.
Int J Syst Evol Microbiol. 2019 Jan;69(1):33-38. doi: 10.1099/ijsem.0.002981. Epub 2018 Nov 8.
Two isolates of a Gram-positive, non-motile, coccoid or oval-shaped anaerobic bacterium, designated strains N6H1-15 and YH1_16, were isolated from faecal samples obtained from a mature dog. Analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Blautia coccoidesrRNA gene group (cluster XIVa) and were closely related to Blautia hansenii KCTC 5951, Blautia stercoris KCTC 5981, Blautia producta producta KCTC 3695 and B. coccoides DSM 15327, with 96.7, 94.4, 94.2 and 93.9 % sequence similarity, respectively. The two isolates contained m-diaminopimelic acid within their peptidoglycans. The major polar lipids were diphosphatidylglycerol and phosphatidylglycerol, and the major fatty acids were C16 : 0 (18.5 %), C16 : 0 (18.0 %) and C18 : 1cis 9 (16.2 %). The predominant metabolic end products of glucose fermentation were acetic and lactic acids, and the G+C content was 44.2 mol%. Thus, the polyphasic data suggest that the two new isolates represent a new species, proposed as Blautia argi sp. nov. The type strain is N6H1-15 (=KCTC 15426=JCM 31394).
从一只成年犬的粪便样本中分离出两株革兰氏阳性、无运动性、球状或椭圆形的厌氧细菌，分别命名为菌株N6H1-15和YH1_16。16S rRNA基因序列分析表明，这些分离株属于布劳特氏菌属球形rRNA基因组（第十四a簇），与汉氏布劳特氏菌KCTC 5951、粪便布劳特氏菌KCTC 5981、产酸布劳特氏菌产酸亚种KCTC 3695和球形布劳特氏菌DSM 15327密切相关，序列相似性分别为96.7%、94.4%、94.2%和93.9%。这两株分离株的肽聚糖中含有间二氨基庚二酸。主要极性脂为双磷脂酰甘油和磷脂酰甘油，主要脂肪酸为C16 : 0（18.5 %）、C16 : 0（18.0 %）和C18 : 1顺式9（16.2 %）。葡萄糖发酵的主要代谢终产物是乙酸和乳酸，G+C含量为44.2mol%。因此，多相数据表明这两株新分离株代表一个新物种，提议命名为阿氏布劳特氏菌（Blautia argi sp. nov.）。模式菌株为N6H1-15（=KCTC 15426=JCM 31394）。
