Paek Jayoung, Shin Yeseul, Kim Joong-Su, Kim Hongik, Kook Joong-Ki, Paek Woon Kee, Chang Young-Hyo
ABS Research Support Center, KRIBB, 125 Gwahak-ro, Yuseong-gu, Daejeon 305-806, Republic of Korea.
Jeonbuk Branch Institute, Molecular Bioprocess Research Center, KRIBB, 1404 Sinjeong-dong, Jeongeup-si, Jeonbuk 580-185, Republic of Korea.
Anaerobe. 2017 Dec;48:70-75. doi: 10.1016/j.anaerobe.2017.07.006. Epub 2017 Jul 25.
Gram-positive, straight or slightly curved rod-shaped bacteria, designated as strains N6H1-5 and N6H1-3, were isolated from fecal samples of old dog. The analysis of 16S rRNA gene sequences indicated that the isolates belonged to the Clostridium cluster XVI and were closely related to Eubacterium dolichum KCTC 5832, Eubacterium tortuosum DSM 3987, Clostridium innocuum KCTC 5183, Allobaculum stecoricanis DSM 13633, Eubacterium limosum KCTC 3266, and Clostridium butyricum KCTC 1871, with 94.0%, 93.8%, 92.0%, 84.9%, 80.7%, and 80.0% sequence similarity, respectively. Chemotaxonomic data supported placement of the strains N6H1-5 and N6H1-3 in the new taxon. The strains contained m-diaminopimelic acid cell wall peptidoglycan; the major polar lipids were diphosphatidylglycerol (DPG), phosphatidylglycerol (PG), and glycolipids (GL); and the major fatty acids were Ccis 9 (30.7%) and C (17.1%). The predominant metabolic end product was lactic acid. The G + C content was 35.8 mol%. The most closely related species, E. dolichum and E. tortuosum, were also assigned to the new taxon, based on the phylogenetic analysis and phenotypic data. Thus, the type strain N6H1-5 (=KCTC 15422 = JCM 30884) represents a novel genus and species, for which the name Absiella argi gen. nov., sp. nov is proposed. It is also proposed that E. dolichum KCTC 5832 and E. tortuosum DSM 3987 be transferred to this new genus, and named Absiella dolichum comb. nov. and Absiella tortuosum comb. nov., respectively.
从一只老年犬的粪便样本中分离出革兰氏阳性、直或稍弯曲的杆状细菌，分别命名为菌株N6H1-5和N6H1-3。16S rRNA基因序列分析表明，这些分离株属于梭菌属第十六簇，与多瘤真杆菌KCTC 5832、弯曲真杆菌DSM 3987、无害梭菌KCTC 5183、粪便嗜杆菌DSM 13633、黏液真杆菌KCTC 3266和丁酸梭菌KCTC 1871密切相关，序列相似性分别为94.0%、93.8%、92.0%、8由.9%、80.7%和80.0%。化学分类学数据支持将菌株N6H1-5和N6H1-3归入新分类单元。这些菌株含有间二氨基庚二酸细胞壁肽聚糖；主要极性脂为双磷脂酰甘油（DPG）、磷脂酰甘油（PG）和糖脂（GL）；主要脂肪酸为顺式9（30.7%）和17.1%。主要代谢终产物是乳酸。G + C含量为35.8 mol%。基于系统发育分析和表型数据，最密切相关的物种多瘤真杆菌和弯曲真杆菌也被归入新分类单元。因此，模式菌株N6H1-5（=KCTC 15422 = JCM 30884）代表一个新的属和种，为此提出新名称阿氏菌属阿氏菌（Absiella argi gen. nov., sp. nov）。还建议将多瘤真杆菌KCTC 5832和弯曲真杆菌DSM 3987转移到这个新属，分别命名为多瘤阿氏菌（Absiella dolichum comb. nov）和弯曲阿氏菌（Absiella tortuosum comb. nov）。
