Seo Boram, Yoo Ju Eun, Lee Yung Mi, Ko GwangPyo
Department of Environmental Health Sciences, Graduate School of Public Health, Seoul National University, Seoul, Republic of Korea.
School of Biological Sciences, Seoul National University, Seoul, Republic of Korea.
Int J Syst Evol Microbiol. 2017 Jul;67(7):2430-2435. doi: 10.1099/ijsem.0.001977. Epub 2017 Jul 26.
A strictly anaerobic Gram-stain-positive, non-motile and non-spore-forming bacterial strain, BR31T, was isolated from a faecal sample of a healthy human. Bacterial colonies were ivory-coloured on GAM agar and composed of rod-shaped cells with rounded ends approximately 1.4-2.1×0.5-0.6 µm in size. According to comparative analysis based on 16S rRNA gene sequences, strain BR31T formed a distinct phylogenetic lineage that reflects a new genus within Clostridium cluster XIVa in the family Lachnospiraceae, with highest similarity to Eubacterium contortum DSM 3982T (94.6 %). The DNA G+C content was calculated to be 47.0 mol% from whole genome sequencing. Predicted genes associated with synthesis of teichuronic acid, polyamines, polar lipids and diaminopimelic acid were detected. The peptidoglycan contained meso-diaminopimelic acid and the main polar lipid detected was phosphatidylglycerol. The major metabolic end product of glucose was acetic acid, which was in agreement with those of most members of the family. However, the profile of major cellular fatty acids (C16 : 0, C14 : 0, summed feature 4 and C13 : 0) and overall enzyme activity demonstrated phenotypic differentiation of strain BR31T from other closely related genera. Thus, based on distinct phenotypic, phylogenetic, genotypic and chemotaxonomic characteristics, strain BR31T is considered to represent a novel species of a new genus, for which the name Merdimonas faecis gen. nov., sp. nov. is proposed. The type strain of Merdimonas faecis is BR31T (=KCTC 15482T=JCM 30748T).
从一名健康人的粪便样本中分离出一株严格厌氧、革兰氏染色阳性、不运动且不形成芽孢的细菌菌株BR31T。该细菌在GAM琼脂上的菌落呈象牙色，由两端圆形的杆状细胞组成，大小约为1.4 - 2.1×0.5 - 0.6 µm。基于16S rRNA基因序列的比较分析表明，菌株BR31T形成了一个独特的系统发育谱系，反映了毛螺菌科梭菌属簇XIVa中的一个新属，与扭曲真杆菌DSM 3982T的相似度最高（94.6%）。通过全基因组测序计算得出DNA G + C含量为47.0 mol%。检测到与磷壁酸、多胺、极性脂质和二氨基庚二酸合成相关的预测基因。肽聚糖含有内消旋二氨基庚二酸，检测到的主要极性脂质是磷脂酰甘油。葡萄糖的主要代谢终产物是乙酸，这与该科大多数成员的情况一致。然而，主要细胞脂肪酸（C16 : 0、C14 : 0、总和特征4和C13 : 0）的谱图以及总体酶活性表明菌株BR31T与其他密切相关属在表型上存在差异。因此，基于独特的表型、系统发育、基因型和化学分类学特征，菌株BR31T被认为代表了一个新属的新物种，为此提出了新属名粪栖单胞菌属（Merdimonas）、新种名粪栖单胞菌（Merdimonas faecis）。粪栖单胞菌的模式菌株是BR31T（=KCTC 15482T = JCM 30748T）。
