School of Pharmacy, University of Waterloo, Waterloo ON, N2L 3G1, Canada; Department of Pharmaceutics, Faculty of Pharmacy, Jazan University, Jizan, Saudi Arabia.
School of Pharmacy, University of Waterloo, Waterloo ON, N2L 3G1, Canada; Department of Pharmaceutics and Industrial Pharmacy, Faculty of Pharmacy, Ain Shams University, Cairo, Egypt.
Int J Pharm. 2019 Jan 10;554:224-234. doi: 10.1016/j.ijpharm.2018.11.001. Epub 2018 Nov 5.
Gelatin nanoparticles (GNPs) are one of the most extensively used natural polymers for gene therapy. With advantages of being biodegradable, biocompatible, low cost and easily modified, gelatin holds great promise as a non-viral system for gene delivery. This review examines various methods of preparation of modified gelatin nanoparticles and considers how these modifications apply to gene delivery. The article discusses cationic gelatin, PEGylated gelatin, thiolated gelatin, alendronate gelatin, and EGFR gelatin nanoparticles. This article also considers several advantages of these modifications and their contribution to the improvement in the efficiency of these systems, resulting in superior transfection and enhanced gene delivery in general.
明胶纳米粒（GNPs）是基因治疗中最广泛使用的天然聚合物之一。明胶具有可生物降解、生物相容性、低成本和易于修饰等优点，因此作为非病毒基因传递系统具有很大的应用前景。本综述考察了制备改性明胶纳米粒的各种方法，并考虑了这些修饰方法如何应用于基因传递。文章讨论了阳离子明胶、聚乙二醇化明胶、巯基化明胶、阿仑膦酸钠明胶和 EGFR 明胶纳米粒。本文还考虑了这些修饰的几个优点及其对提高这些系统效率的贡献，从而导致转染效率提高和基因传递增强。
