Edrisi Maryan Khazar, Samizadeh Lahiji Habibollah, Farrokhi Naser, Hasani Komeleh Hassan
Department of Plant Biotechnology, Faculty of Agriculture, University of Guilan, Rasht, Iran.
Department of Plant Biotechnology, Faculty of Agriculture, University of Guilan, Rasht, Iran.
Gene Expr Patterns. 2019 Jan;31:7-17. doi: 10.1016/j.gep.2018.10.002. Epub 2018 Nov 6.
Dehydrins (DHNs) are plant specific cold and drought stress-responsive proteins that belong to late embryogenesis abundant (LEA) protein families. B. napus DHNs (BnDHNs) were computationally analyzed to establish gene regulatory- and protein-protein interaction networks. Promoter analyses suggested functionality of phytohormones in BnDHNs gene network. The relative expressions of some BnDHNs were analyzed using qRT-PCR in seedling leaves of both cold-tolerant (Zarfam) and -sensitive (Sari Gul) canola treated/untreated by cold. Our expression data were indicative of the importance of BnDHNs in cold tolerance in Zarfam. BnDHNs were classified into three classes according to the expression pattern. Moreover, expression of three BnDHN types, SK (BnLEA10 and BnLEA18), YK (BnLEA90) and YSK (BnLEA104) were significantly high in the tolerant cultivar at 12 h of cold treatment. Our findings put forward the possibility of considering these genes as screening biomarker to determine cold-tolerant breeding lines; something that needs to be further corroborated. Furthermore, these genes may have some implications in developing such tolerant lines via transgenesis.
脱水蛋白（DHNs）是植物特有的、对寒冷和干旱胁迫有响应的蛋白质，属于胚胎发育晚期丰富（LEA）蛋白家族。对甘蓝型油菜脱水蛋白（BnDHNs）进行了计算分析，以建立基因调控和蛋白质-蛋白质相互作用网络。启动子分析表明植物激素在BnDHNs基因网络中具有功能。使用qRT-PCR分析了耐寒（扎尔法姆）和冷敏感（萨里·古尔）油菜在冷处理/未冷处理的幼苗叶片中一些BnDHNs的相对表达。我们的表达数据表明BnDHNs在扎尔法姆的耐寒性中具有重要作用。根据表达模式，BnDHNs被分为三类。此外，在冷处理12小时时，三种BnDHN类型，即SK（BnLEA10和BnLEA18）、YK（BnLEA90）和YSK（BnLEA104）在耐冷品种中的表达显著较高。我们的研究结果提出了将这些基因作为筛选生物标志物以确定耐寒育种系的可能性；这一点需要进一步证实。此外，这些基因可能在通过转基因培育此类耐冷品系方面具有一些意义。
