Department of Production Engineering and Plant Genetics, Faculty of Agriculture, Shahid Chamran University of Ahvaz, P.O. Box 61355/144, Ahvaz, Iran.
Department of European and Mediterranean Cultures, Architecture, Environment, Cultural Heritage (DiCEM), Università degli Studi della Basilicata, Via Lanera 20, 75100, Matera, MT, Italy.
BMC Plant Biol. 2024 Mar 20;24(1):206. doi: 10.1186/s12870-024-04893-0.
Plants mediate several defense mechanisms to withstand abiotic stresses. Several gene families respond to stress as well as multiple transcription factors to minimize abiotic stresses without minimizing their effects on performance potential. RNA helicase (RH) is one of the foremost critical gene families that can play an influential role in tolerating abiotic stresses in plants. However, little knowledge is present about this protein family in rapeseed (canola). Here, we performed a comprehensive survey analysis of the RH protein family in rapeseed (Brassica napus L.).
A total of 133 BnRHs genes have been discovered in this study. By phylogenetic analysis, RHs genes were divided into one main group and a subgroup. Examination of the chromosomal position of the identified genes showed that most of the genes (27%) were located on chromosome 3. All 133 identified sequences contained the main DEXDC domain, the HELICC domain, and a number of sub-domains. The results of biological process studies showed that about 17% of the proteins acted as RHs, 22% as ATP binding, and 14% as mRNA binding. Each part of the conserved motifs, communication network, and three-dimensional structure of the proteins were examined separately. The results showed that the RWC in leaf tissue decreased with higher levels of drought stress and in both root and leaf tissues sodium concentration was increased upon increased levels of salt stress treatments. The proline content were found to be increased in leaf and root with the increased level of stress treatment. Finally, the expression patterns of eight selected RHs genes that have been exposed to drought, salinity, cold, heat and cadmium stresses were investigated by qPCR. The results showed the effect of genes under stress. Examination of gene expression in the Hayola #4815 cultivar showed that all primers except primer #79 had less expression in both leaves and roots than the control level.
New finding from the study have been presented new insights for better understanding the function and possible mechanism of RH in response to abiotic stress in rapeseed.
植物具有多种防御机制来抵御非生物胁迫。一些基因家族会对胁迫做出反应，同时也有多个转录因子来最小化非生物胁迫对性能潜力的影响。RNA 解旋酶（RH）是最重要的关键基因家族之一，它可以在植物耐受非生物胁迫方面发挥重要作用。然而，人们对油菜（甘蓝型油菜）中的这种蛋白质家族知之甚少。本研究对油菜中的 RH 蛋白家族进行了全面的调查分析。
本研究共发现 133 个 BnRH 基因。通过系统发育分析，RH 基因分为一个主群和一个亚群。鉴定基因的染色体位置分析表明，大多数基因（27%）位于第 3 号染色体上。所有鉴定的序列都包含主要的 DEXDC 结构域、HELICC 结构域和一些亚结构域。生物学过程研究结果表明，约 17%的蛋白质作为 RH 发挥作用，22%作为 ATP 结合，14%作为 mRNA 结合。单独检查了保守基序、通信网络和蛋白质三维结构的各个部分。结果表明，叶片组织中的 RWC 随干旱胁迫水平的升高而降低，在根和叶片组织中，盐胁迫处理水平升高时钠离子浓度升高。随着胁迫处理水平的升高，发现脯氨酸含量在叶片和根中增加。最后，通过 qPCR 研究了 8 个选定的 RH 基因在干旱、盐、冷、热和镉胁迫下的表达模式。结果表明了基因在胁迫下的作用。对 Hayola #4815 品种的基因表达研究表明，除引物 #79 外，所有引物在叶片和根中的表达水平均低于对照水平。
本研究提出了新的发现，为更好地理解 RH 在油菜响应非生物胁迫中的功能和可能机制提供了新的见解。
