ANGPTL8 对 HDL 介导的胆固醇外排能力的负面影响。
The negative effect of ANGPTL8 on HDL-mediated cholesterol efflux capacity.
机构信息
Department of Cardiovascular Medicine, The Second Xiangya Hospital, Central South University, No.139, Middle Renmin Road, Changsha, 410011, Hunan, China.
Department of Metabolism & Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan, China.
出版信息
Cardiovasc Diabetol. 2018 Nov 8;17(1):142. doi: 10.1186/s12933-018-0785-x.
BACKGROUND
It is well known that angiopoietin-like protein 8 (ANGPTL8) exerts its effects on lipid metabolism through the inhibition of lipoprotein lipase and subsequent elevation of plasma triglyceride. However, it is not clear whether ANGPTL8 could affect lipid metabolism via other pathways. The study was aimed to investigate the effects of ANGPTL8 on the function of high-density lipoprotein (HDL), which plays a protective role in atherosclerosis progression.
METHODS
Two hundred and ten subjects were recruited. Plasma ANGPTL8 was measured by enzyme-linked immunosorbent assays. Cholesterol efflux capacity was chosen as the biomarker of HDL function and measured via H-cholesterol loading THP-1 cell models.
RESULTS
ANGPTL8 exhibited no significant difference between CAD group and nonCAD group, but ANGPTL8 in DM group was significantly higher than that in the nonDM group [568.3 (406.2-836.8) vs 458.2 (356.8-755.6), P = 0.023]. Compared to controls, subjects in CAD group and DM group exhibited significantly lower cholesterol efflux capacity [CAD: 14.58 ± 2.06 vs 12.51 ± 2.83%, P < 0.0001; DM: 13.62 ± 2.57 vs 12.34 ± 3.16%, P = 0.0099]. ANGPTL8 was inversely correlated with cholesterol efflux capacity (r = - 0.188, P < 0.01). Regression analysis revealed that plasma ANGPTL8 was an independent contributor to cholesterol efflux capacity (standardized β = - 0.143, P = 0.023).
CONCLUSION
ANGPTL8 presents a negative effect on HDL-mediated cholesterol efflux capacity.
背景
众所周知，血管生成素样蛋白 8（ANGPTL8）通过抑制脂蛋白脂肪酶并随后升高血浆甘油三酯来发挥其对脂质代谢的作用。然而，ANGPTL8 是否可以通过其他途径影响脂质代谢尚不清楚。本研究旨在研究 ANGPTL8 对高密度脂蛋白（HDL）功能的影响，HDL 在动脉粥样硬化进展中起保护作用。
方法
招募了 210 名受试者。通过酶联免疫吸附测定法测量血浆 ANGPTL8。胆固醇外排能力被选为 HDL 功能的生物标志物，并通过 H-胆固醇加载 THP-1 细胞模型进行测量。
结果
ANGPTL8 在 CAD 组和非 CAD 组之间无显著差异，但 DM 组中的 ANGPTL8 明显高于非 DM 组[568.3（406.2-836.8）比 458.2（356.8-755.6），P=0.023]。与对照组相比，CAD 组和 DM 组的胆固醇外排能力明显降低[CAD：14.58±2.06 比 12.51±2.83%，P<0.0001；DM：13.62±2.57 比 12.34±3.16%，P=0.0099]。ANGPTL8 与胆固醇外排能力呈负相关（r=-0.188，P<0.01）。回归分析显示，血浆 ANGPTL8 是胆固醇外排能力的独立贡献者（标准化β=-0.143，P=0.023）。
结论
ANGPTL8 对 HDL 介导的胆固醇外排能力有负面影响。
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