Inoue Daisuke, Kabata Tamon, Kajino Yoshitomo, Shirai Toshiharu, Tsuchiya Hiroyuki
Department of Orthopaedic Surgery, Graduate School of Medical Sciences, Kanazawa University, Japan.
Department of Orthopaedic Surgery, Graduate School of Medical Sciences, Kanazawa University, Japan; Department of Orthopaedic Surgery, Graduate School of Medical Sciences, Kyoto Prefectural University of Medicine, Japan.
J Orthop Sci. 2019 May;24(3):548-551. doi: 10.1016/j.jos.2018.10.010. Epub 2018 Nov 5.
We have developed iodine-supported titanium implants, which were shown to have good anti-bacterial effects for Methicillin-sensitive Staphylococcus aureus (MSSA) in our past basic research. However, PJI can be caused by various bacteria including MRSA, Pseudomonas aeruginosa, MSSE, and fungus. The purpose of this study was to investigate whether these implants also have good antibacterial attachment effects for MRSA, P. aeruginosa, MSSE, and fungus.
Ti-6Al-4V titanium plates were either left untreated (Ti), treated with oxide film on the Ti surface by anodization (Ti-O), or treated with an iodine coating on oxidation film (Ti-I). The antibacterial activity of the TiI was measured by experimental methods according to Japanese Industrial Standard (JIS) protocols. Implants in this study were exposed to MRSA (ATCC43300), P. aeruginosa (ATCC27853), MSSE (ATCC35984), and Candida Albicans (ATCC10231). Colonies were counted immediately after the bacteria attached to the metal surface and again after 24 h incubation. The difference in the number of bacteria on each metal plate was statistically investigated and an antibacterial activity value was calculated. An effective antibacterial active value of more than 2.0 was judged to be effective according to JIS protocol.
No countable viable bacteria were observed on the Ti-I surface. For all bacteria there was a significant difference in the mean number of viable bacteria between Ti-I and Ti or Ti-O. Antibacterial activity value in Ti-I and Ti-O was more than 5.9 and 3.6 respectively for MRSA, more than 2.8 and zero for P. aeruginosa, more than 4.3 and zero for MSSE, and more than 4.7 and zero for C. Albicans.
This study showed that iodine-supported titanium implants have good antimicrobial attachment effects for MRSA, P. aeruginosa, MSSE, and C. Albicans. Iodine-supported titanium implants could have great potential as innovative antibacterial implants that can prevent early onset periprosthetic joint infection.
我们研发了碘负载钛植入物，在过去的基础研究中已证明其对甲氧西林敏感金黄色葡萄球菌（MSSA）具有良好的抗菌效果。然而，假体周围感染（PJI）可由包括耐甲氧西林金黄色葡萄球菌（MRSA）、铜绿假单胞菌、表皮葡萄球菌（MSSE）和真菌在内的多种细菌引起。本研究的目的是调查这些植入物对MRSA、铜绿假单胞菌、MSSE和真菌是否也具有良好的抗菌附着效果。
将Ti-6Al-4V钛板不作处理（Ti）、通过阳极氧化在钛表面形成氧化膜（Ti-O）或在氧化膜上进行碘涂层处理（Ti-I）。根据日本工业标准（JIS）协议，通过实验方法测量Ti-I的抗菌活性。本研究中的植入物暴露于MRSA（ATCC43300）、铜绿假单胞菌（ATCC27853）、MSSE（ATCC35984）和白色念珠菌（ATCC10231）。细菌附着于金属表面后立即计数菌落，并在孵育24小时后再次计数。对每个金属板上的细菌数量差异进行统计学研究，并计算抗菌活性值。根据JIS协议，抗菌活性有效值大于2.0被判定为有效。
在Ti-I表面未观察到可计数的活菌。对于所有细菌，Ti-I与Ti或Ti-O之间的活菌平均数存在显著差异。Ti-I和Ti-O对MRSA的抗菌活性值分别大于5.9和3.6，对铜绿假单胞菌分别大于2.8和0，对MSSE分别大于4.3和0，对白色念珠菌分别大于4.7和0。
本研究表明，碘负载钛植入物对MRSA、铜绿假单胞菌、MSSE和白色念珠菌具有良好的抗菌附着效果。碘负载钛植入物作为可预防早期假体周围关节感染的创新抗菌植入物具有巨大潜力。
