Chandra Shekar B R, Nagarajappa Ramesh, Jain Richa, Singh Rupal, Suma S, Thakur Rupesh
Department of Public Health Dentistry, JSS Dental College and Hospital, Jagadguru Sri Shivarathreeshwara University, Mysore, Karnataka, India.
Department of Public Health Dentistry, Institute of Dental Sciences, Bhubaneshwar, Odisha, India.
Indian J Dent Res. 2018 Sep-Oct;29(5):641-645. doi: 10.4103/ijdr.IJDR_52_17.
The herbal extracts have been effectively tried in the treatment and prevention of many oral diseases.
The aim is to assess the antimicrobial efficacy of Acacia nilotica, Murraya koenigii L. Sprengel, Eucalyptus hybrid, Psidium guajava extracts and their combinations on Fusobacterium nucleatum (Fn) and Porphyromonas gingivalis (Pg).
The extraction process was carried out by Soxhlet apparatus using ethanol as solvent. The combinations of the four plant extracts were prepared by combining an equal quantity of 10% solution of each of the four plant extracts. The antimicrobial efficacy testing of the plant extracts and their combinations on Fn and Pg was performed using agar well diffusion method. Columbia 5% of sheep blood agar plates were used for antimicrobial efficacy testing under anaerobic conditions. The qualitative assay was carried out to identify the various phytochemical constituents. Dimethyl sulfoxide and 0.2% chlorhexidine acted as negative and positive controls, respectively. The mean diameter of inhibition zone between different categories was compared using one-way analysis of variance.
All the individual plant extracts and their double, triple, and quadruple combinations were effective in inhibiting the growth of these bacteria. However, 0.2% chlorhexidine produced the highest mean diameter of inhibition zone.
The plant extracts in combinations offer enhanced antimicrobial efficacy due to their synergistic action besides slowing the development of bacterial resistance. Hence, these extracts in combinations could be used tried as effective alternates to chlorhexidine.
草药提取物已在多种口腔疾病的治疗和预防中得到有效尝试。
评估阿拉伯胶树、九里香、杂交桉、番石榴提取物及其组合对具核梭杆菌（Fn）和牙龈卟啉单胞菌（Pg）的抗菌效果。
采用索氏提取器，以乙醇为溶剂进行提取。将四种植物提取物各10%的溶液等量混合，制备四种植物提取物的组合。采用琼脂孔扩散法对植物提取物及其组合对Fn和Pg进行抗菌效果测试。在厌氧条件下，使用含5%绵羊血的哥伦比亚琼脂平板进行抗菌效果测试。进行定性分析以鉴定各种植物化学成分。二甲基亚砜和0.2%洗必泰分别作为阴性和阳性对照。使用单因素方差分析比较不同类别之间抑菌圈的平均直径。
所有单一植物提取物及其双重、三重和四重组合均能有效抑制这些细菌的生长。然而，0.2%洗必泰产生的抑菌圈平均直径最大。
植物提取物组合由于其协同作用，除了减缓细菌耐药性的发展外，还具有增强的抗菌效果。因此，这些提取物组合可尝试作为洗必泰的有效替代品。
