Chandra Shekar B R, Nagarajappa Ramesh, Jain Richa, Singh Rupal, Thakur Rupesh, Shekar Suma
Ph.D Scholar, Faculty of Dental Sciences, Pacific Academy of Higher Education and Research University, Udaipur, Rajasthan, India.
Department of Public Health Dentistry, Rama Dental College, Khanpur, Uttar Pradesh, India.
Dent Res J (Isfahan). 2016 Mar-Apr;13(2):168-73. doi: 10.4103/1735-3327.178206.
The aim of this in vitro study was to assess antimicrobial efficacy of Acacia nilotica, Murraya koenigii (L.) Sprengel, Eucalyptus hybrid, Psidium guajava extracts, and their combination on Streptococcus mutans and Lactobacillus acidophilus.
The branches of four plants were collected, identified, and authenticated by a taxonomist. The plants were rinsed in water, healthy leaves were separated and shade dried over a period of 3-4 weeks. Soxhlet apparatus using ethanol was employed for extraction procedure. The combinations of plant extracts were prepared by mixing equal quantities of 10% solutions of each of these extracts. 0.2% chlorhexidine and dimethyl sulfoxide were used as positive and negative controls, respectively. The antimicrobial efficacy testing was done using agar well-diffusion method under anaerobic conditions. The mean diameter of inhibition zone was computed and compared between different categories using one-way analysis of variance and Tukey's post-hoc test. A qualitative assay was carried out to identify the various phytochemical constituents in the plants. The data was assessed by SPSS version 20. The statistical significance was fixed at 0.05.
All the plants extracts and their combinations inhibited S. mutans and L. acidophilus. However, the quadruple combination of A. nilotica + M. koenigii (L.) Sprengel + Eucalyptus hybrid + P. guajava produced the maximum inhibition zone (23.5 ± 2.2 mm) against S. mutans. Although, 0.2% chlorhexidine produced the highest inhibition zone against L. acidophilus (18.8 ± 1.2 mm), A. nilotica extract produced maximum inhibition among the various plant extracts and their combinations (14.1 ± 1.8 mm).
All the individual plant extracts and their combinations were effective against S. mutans and L. acidophilus. These could be tried as herbal alternates to chlorhexidine. However, these in vitro results have to be further evaluated for any toxicity of the polyherbal combinations in animal models and effectiveness has to be assessed using in vivo studies on humans.
本体外研究的目的是评估阿拉伯胶树、九里香、杂交桉、番石榴提取物及其组合对变形链球菌和嗜酸乳杆菌的抗菌效果。
采集四种植物的枝条，由分类学家进行鉴定和认证。将植物在水中冲洗，分离出健康的叶子，并在3 - 4周内阴干。采用索氏提取器用乙醇进行提取。通过混合等量的每种提取物的10%溶液来制备植物提取物的组合。分别使用0.2%氯己定和二甲基亚砜作为阳性和阴性对照。在厌氧条件下采用琼脂孔扩散法进行抗菌效果测试。计算抑菌圈的平均直径，并使用单因素方差分析和Tukey事后检验在不同类别之间进行比较。进行定性分析以鉴定植物中的各种植物化学成分。数据采用SPSS 20版进行评估。统计学显著性设定为0.05。
所有植物提取物及其组合均能抑制变形链球菌和嗜酸乳杆菌。然而，阿拉伯胶树 + 九里香 + 杂交桉 + 番石榴的四联组合对变形链球菌产生的抑菌圈最大（23.5 ± 2.2毫米）。虽然0.2%氯己定对嗜酸乳杆菌产生的抑菌圈最大（18.8 ± 1.2毫米），但阿拉伯胶树提取物在各种植物提取物及其组合中产生的抑菌作用最大（14.1 ± 1.8毫米）。
所有单一植物提取物及其组合对变形链球菌和嗜酸乳杆菌均有效。这些可作为氯己定的草药替代品进行尝试。然而，这些体外结果必须在动物模型中进一步评估多草药组合的任何毒性，并且必须通过对人类的体内研究来评估其有效性。
