Demetrescu Ioana, Dumitriu Cristina, Totea Georgeta, Nica Cristina I, Dinischiotu Anca, Ionita Daniela
Faculty of Applied Chemistry and Materials Science POLITEHNICAof Bucharest, Romania Str. Polizu1-7, 011061 Bucharest, Romania.
Faculty of Biomedical Engineering POLITEHNICA of Bucharest, Romania Str. Polizu1-7, 011061 Bucharest, Romania.
Pharmaceutics. 2018 Nov 8;10(4):220. doi: 10.3390/pharmaceutics10040220.
The present paper aims atincreasing the bioperformance of implantable Ti50Zr alloy using zwitterionic cysteine drug coating. Aspects such as stability, biocompatibility, and antibacterial effects were investigated with the help of various methods such as infrared spectroscopy (FT-IR), scanning electronic microscopy (SEM), electrochemical methods, contact angle determinations and cell response. The experimental data of zwitterionic cysteine coating indicate the existence of a hydration layer due to hydrophilic groups evidenced in FT-IR which is responsible for the decrease of contact angle and antibacterial capabilities. The electrochemical stability was evaluatedbased on Tafel plots and electrochemical impedance spectroscopy (EIS). The cell response to cysteine was determined with gingival fibroblasts measuring lactate dehydrogenase (LDH) activity, concentrations of nitric oxide (NO) and intracellular level of reactive oxygen species (ROS). All experimental results supported the increase of stability and better cells response of implantable Ti50Zr alloy coated with zwitterionic cysteine drug. The antibacterial index was measured against and . It was demonstrated that the coating enhanced the production of intracellular ROS in time, which subsequently caused a significant increase in antibacterial index.
本文旨在通过两性离子半胱氨酸药物涂层提高可植入Ti50Zr合金的生物性能。借助傅里叶变换红外光谱（FT-IR）、扫描电子显微镜（SEM）、电化学方法、接触角测定和细胞反应等各种方法，研究了稳定性、生物相容性和抗菌效果等方面。两性离子半胱氨酸涂层的实验数据表明，由于FT-IR中显示的亲水性基团，存在一个水合层，这导致了接触角的减小和抗菌能力。基于塔菲尔曲线和电化学阻抗谱（EIS）评估了电化学稳定性。通过测量牙龈成纤维细胞中乳酸脱氢酶（LDH）活性、一氧化氮（NO）浓度和细胞内活性氧（ROS）水平，确定了细胞对半胱氨酸的反应。所有实验结果均支持涂覆两性离子半胱氨酸药物的可植入Ti50Zr合金稳定性的提高和更好的细胞反应。测定了针对[具体菌种1]和[具体菌种2]的抗菌指数。结果表明，该涂层能及时增强细胞内ROS的产生，随后导致抗菌指数显著增加。
