Ong Kuan Shion, Cheow Yuen Lin, Lee Sui Mae
School of Science, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor, Malaysia.
Tropical Medicine and Biology Multidisciplinary Platform, Monash University Malaysia, Jalan Lagoon Selatan, 47500 Bandar Sunway, Selangor, Malaysia.
J Adv Res. 2017 Jul;8(4):393-398. doi: 10.1016/j.jare.2017.05.007. Epub 2017 May 21.
The increase in prevalence of antimicrobial-resistant bacteria (ARB) is currently a serious threat, thus there is a need for new antimicrobial compounds to combat infections caused by these ARB. An antimicrobial-producing bacterium, was recently isolated and was able to produce a type of siderophore with antimicrobial properties, later identified as pyochelin. The chelating ability of pyochelin has been well-characterized but not for its antimicrobial characteristics. It was found that pyochelin had MIC values (MBC values) of 3.13 µg/mL (6.26 µg/mL) and 6.26 µg/mL (25.00 µg/mL) against three strains and four strains. Pyochelin was able to inhibit ATCC 700802 (a vancomycin-resistant strain) in a time and dose dependent manner via killing kinetics assay. It was demonstrated that pyochelin enhanced the production of intracellular reactive oxygen species (ROS) over time, which subsequently caused a significant increase in malondialdehyde (MDA) production (a marker for lipid peroxidation) and ultimately led to cell death by disrupting the integrity of the bacterial membrane (validated via BacLight assay). This study has revealed the mechanism of action of pyochelin as an antimicrobial agent for the first time and has shown that pyochelin might be able to combat infections caused by in the future.
抗菌耐药菌（ARB）患病率的增加目前是一个严重威胁，因此需要新的抗菌化合物来对抗由这些ARB引起的感染。最近分离出一种产生抗菌物质的细菌，它能够产生一种具有抗菌特性的铁载体，后来被鉴定为绿脓菌素。绿脓菌素的螯合能力已得到充分表征，但其抗菌特性尚未得到充分研究。研究发现，绿脓菌素对三株 菌株和四株 菌株的最低抑菌浓度（最低杀菌浓度）分别为3.13 µg/mL（6.26 µg/mL）和6.26 µg/mL（25.00 µg/mL）。通过杀菌动力学试验，绿脓菌素能够以时间和剂量依赖的方式抑制耐万古霉素菌株ATCC 700802。结果表明，随着时间的推移，绿脓菌素会增强细胞内活性氧（ROS）的产生，随后导致丙二醛（MDA）产量显著增加（脂质过氧化的标志物），最终通过破坏细菌膜的完整性导致细胞死亡（通过BacLight试验验证）。这项研究首次揭示了绿脓菌素作为抗菌剂的作用机制，并表明绿脓菌素未来可能能够对抗由 引起的感染。
