Zimmerman S B, Trach S O
Laboratory of Molecular Biology, National Institutes of Diabetes and Digestive and Kidney Diseases, Bethesda, MD 20892.
Nucleic Acids Res. 1988 Jul 25;16(14A):6309-26. doi: 10.1093/nar/16.14.6309.
The equilibrium for the binding reaction between the 30 S and 50 S ribosomal subunits of E. coli is shifted towards formation of 70 S ribosomes in the presence of a variety of polymers. The polymers also increase a further interaction between 70 S particles to form the 100 S dimer. The requirement for relatively high concentrations of non-specific polymers indicates that the shifts in equilibria arise from excluded volume effects. Analysis using scaled particle theory is consistent with this mechanism. The effects of high concentrations of polymers on the interactions between ribosomal species may make important changes in the function of ribosomes under the crowded conditions which occur in vivo.
在多种聚合物存在的情况下，大肠杆菌30 S和50 S核糖体亚基之间结合反应的平衡会向形成70 S核糖体的方向移动。这些聚合物还会增强70 S颗粒之间进一步相互作用以形成100 S二聚体。对相对高浓度非特异性聚合物的需求表明，平衡的移动源于排除体积效应。使用定标粒子理论进行的分析与这一机制相符。高浓度聚合物对核糖体种类间相互作用的影响可能会在体内发生的拥挤条件下使核糖体功能产生重要变化。
