Center Lab of Longhua Branch, Shenzhen People's Hospital, 2nd Clinical Medical College of Jinan University, Shenzhen 518120, China.
Guangdong Key Laboratory for Research and Development of Natural Drugs, Guangdong Medical University, Zhanjiang 524023, China.
Fitoterapia. 2018 Nov;131:236-244. doi: 10.1016/j.fitote.2018.11.005. Epub 2018 Nov 8.
Six previously undescribed naturally occurring meroterpenoids (2, 5-9) together with seven known meroterpenoids (1, 3, 4, 10-13) were isolated from the root plant of Arnebia euchroma. Their structures and absolute configurations were determined by extensive 1D (H NMR, C NMR) and 2D NMR (HH COSY, DEPT, HMQC, HMBC, NOESY) spectroscopic methods, spectroscopy high resolution mass spectrometry, as well as DFT and MM2 force-field calculations. Meroterpenoids 1-13 were evaluated for their cytotoxicities against human liver cancer cell lines SMMC-7721, HepG2, QGY-7703 and HepG2/ADM. Meroterpenoid 5 exhibited the most potent activity with IC values of 6.40 ± 0.51, 3.86 ± 0.28, 3.43 ± 0.27 and 11.31 ± 0.67 μM, respectively. Meroterpenoid 4 exhibited significant growth inhibitory effects against HepG2/ADM with IC at 18.77 ± 1.23 μM, and meroterpenoid 8 with IC at 5.41 ± 0.51 and 6.18 ± 0.47 μM against HepG2 and QGY-7703, respectively. These were more potent than the positive drug, Cisplatin.
从秀丽弯管花的根部分离得到六个以前未描述的天然生源 Meroterpenoids（2、5-9）和七个已知的 Meroterpenoids（1、3、4、10-13）。通过广泛的 1D（H NMR、C NMR）和 2D NMR（HH COSY、DEPT、HMQC、HMBC、NOESY）光谱方法、高分辨率质谱、DFT 和 MM2 力场计算确定了它们的结构和绝对构型。对 Meroterpenoids 1-13 进行了人肝癌细胞系 SMMC-7721、HepG2、QGY-7703 和 HepG2/ADM 的细胞毒性评估。Meroterpenoid 5 显示出最强的活性，IC 值分别为 6.40±0.51、3.86±0.28、3.43±0.27 和 11.31±0.67μM。Meroterpenoid 4 对 HepG2/ADM 具有显著的生长抑制作用，IC 值为 18.77±1.23μM，Meroterpenoid 8 对 HepG2 和 QGY-7703 的 IC 值分别为 5.41±0.51 和 6.18±0.47μM，均强于阳性药物顺铂。
