Laboratory of Neuro-Oncology/Clinical and Cancer Proteomics, Department of Neurology, Erasmus University Medical Center Rotterdam, 3015 CN, Rotterdam, The Netherlands.
Departments of Obstetrics and Gynecology, Erasmus University Medical Center Rotterdam, 3015 CN, Rotterdam, The Netherlands.
Proteomics Clin Appl. 2019 May;13(3):e1800181. doi: 10.1002/prca.201800181. Epub 2018 Nov 26.
The objective of present study is to determine serum levels and placental distribution of two interacting proteins calcyclin and heat shock protein 90 in preeclampsia.
Maternal serum levels of calcyclin and heat shock protein 90 are compared throughout pregnancy from the first trimester till term among women with preeclampsia (n = 43) and age-matched normotensive pregnant controls (n = 46). A serum-based 2D LC-MS assay using Parallel Reaction Monitoring is applied to quantify both calcyclin and heat shock protein 90.
Serum levels of calcyclin are significantly lower in patients with preeclampsia in the second trimester of pregnancy as compared to controls (p < 0.05). Serum levels of heat shock protein 90 are significantly higher in patients with preeclampsia in the third trimester as compared to controls (p < 0.001).
Both interacting proteins calcyclin and heat shock protein 90 are notably changed in preeclamptic patients compared to controls. Calcyclin is already decreased before the onset of preeclampsia in the second trimester and HSP90 is strongly increased in the third trimester. This suggests that these proteins may play a role in the pathogenesis of preeclampsia and ought to be investigated in large cohort studies as molecular biomarkers.
本研究旨在确定子痫前期患者血清中两种相互作用蛋白钙调蛋白和热休克蛋白 90 的水平及其胎盘分布。
比较 43 例子痫前期患者和 46 例年龄匹配的正常血压孕妇在整个孕期（从孕早期到足月）的血清钙调蛋白和热休克蛋白 90 水平。应用基于血清的 2D LC-MS 分析方法，采用平行反应监测定量分析钙调蛋白和热休克蛋白 90。
与对照组相比，子痫前期患者在孕中期血清钙调蛋白水平显著降低（p<0.05）。与对照组相比，子痫前期患者在孕晚期血清热休克蛋白 90 水平显著升高（p<0.001）。
与对照组相比，子痫前期患者的两种相互作用蛋白钙调蛋白和热休克蛋白 90 均明显改变。钙调蛋白在子痫前期的第二孕期之前就已经减少，而 HSP90 在第三孕期则显著增加。这表明这些蛋白可能在子痫前期的发病机制中起作用，应该在大样本队列研究中作为分子生物标志物进行研究。
